LOCUS       sequence-189603-        1854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 6 of EMMA. Functional category: 
            Kozak sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1895 HC_Kan_Kozak-ATG_p6
FEATURES             Location/Qualifiers
     source          1..1854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      193..224
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        225..327
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             328..1143
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(401..420)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1167..1194
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1206..1793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1286..1305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1815..1844)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa tgatatcaag cttgaattcg
       61 ttacgtctcg ccagaaccgt cagatccgct agagattacg ccaaccgcca ccatgggcag
      121 ccgagacgga cgaattctct agatatcgct caatactgac catttaaatc atacctgacc
      181 tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga
      241 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
      301 agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgctcg
      361 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
      421 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
      481 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg
      541 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
      601 gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa
      661 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
      721 cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag
      781 gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat
      841 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
      901 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
      961 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     1021 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     1081 ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc
     1141 taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg
     1201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag
     1261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1681 gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1741 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1801 ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag ttga
//
