LOCUS       sequence-189605-        2415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 9 of EMMA. Functional category: 
            Selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1899 HC_Kan_PuroR_p9
FEATURES             Location/Qualifiers
     source          1..2415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             74..670
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     567..587
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     terminator      759..788
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      810..1397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1298..1317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1409..1436)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1460..2275)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2183..2202
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2276..2378)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2379..2410)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctatggaggt caggtatgat ttaaatggtc agtattgagc gatatctaga gaattcgtca
       61 cgtctcggcg tccaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
      121 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
      181 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
      241 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
      301 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
      361 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
      421 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtg tcgcccgacc accagggcaa
      481 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
      541 cgccttcctg gaaacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
      601 cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc
      661 cggtgcctaa taatgctcga gacgaaaacg aattcaagct tgatatcatt caggacgagc
      721 ctcagactcc agcgtaactg gactgcaatc aactcactgg ctcaccttca cgggtgggcc
      781 tttcttcggt agaaaatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      841 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      901 ccaactcttt ttccgaggta actggcttca gcagagcgca gataccaaat actgttcttc
      961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1321 gtcctgtcgg gtttcgccac ctctgacttg agcatcgatt tttgtgatgc tcgtcagggg
     1381 ggcggagcct atggaaaaac gccagcaacg cagaaaggcc cacccgaagg tgagccaggt
     1441 gattacattt gggccctcat tagaaaaact catcgagcat caagtgaaac tgcaatttat
     1501 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     1561 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     1621 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     1681 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
     1741 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca ccaaccaaac
     1801 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgccg ttaaaaggac
     1861 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1921 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
     1981 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     2041 taaattccgt cagccagttt agcctgacca tctcatctgt aacatcattg gcaacgctac
     2101 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     2161 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     2221 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcattttag
     2281 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2341 cattatggtg aaagttggaa cctcttacgt gccgatcaag tcaaaagcct ccggtcggag
     2401 gcttttgact ttctg
//
