LOCUS       sequence-189606-        2042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 22 of EMMA. Functional category: 
            polyadenylation signal sequence .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1901 HC_Kan_PGKpA_p22
FEATURES             Location/Qualifiers
     source          1..2042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      385..414
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      436..1023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     924..943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1035..1062)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1086..1901)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1809..1828
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1902..2004)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2005..2036)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac
       61 gtctcggttg ccttgagcat ctgacttctg gctaaattga tgatctatta aacaataaag
      121 atgtccacat ggaagttttt tcctgtcata ctttgttaag aagggtgaga acagagtacc
      181 tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat
      241 gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca tagttggatc
      301 gaacgagacg aaaacgaatt caagcttgat atcattcagg acgagcctca gactccagcg
      361 taactggact gcaatcaact cactggctca ccttcacggg tgggcctttc ttcggtagaa
      421 aatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      541 gaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      601 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      661 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      721 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      781 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      841 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      901 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      961 cgccacctct gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1021 aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag ccaggtgatt acatttgggc
     1081 cctcattaga aaaactcatc gagcatcaag tgaaactgca atttattcat atcaggatta
     1141 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     1201 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     1261 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     1321 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
     1381 ggccagccat tacgctcgtc atcaaaatca ctcgcaccaa ccaaaccgtt attcattcgt
     1441 gattgcgcct gagcgagacg aaatacgcga tcgccgttaa aaggacaatt acaaacagga
     1501 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     1561 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
     1621 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     1681 cagtttagcc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     1741 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     1801 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     1861 cgcggcctcg agcaagacgt ttcccgttga atatggctca ttttagcttc cttagctcct
     1921 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1981 ttggaacctc ttacgtgccg atcaagtcaa aagcctccgg tcggaggctt ttgactttct
     2041 gc
//
