LOCUS       sequence-189608-        2140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 21 of EMMA. Functional category: 
            Dimerization domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1905 HC_Kan_DmrA_p21
FEATURES             Location/Qualifiers
     source          1..2140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..398
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     terminator      487..516
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      538..1125
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1026..1045
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1137..1164)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1188..2003)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1911..1930
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2004..2106)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2107..2138)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctgctatgga ggtcaggtat gatttaaatg gtcagtattg agcgatatct agagaattcg
       61 tcacgtctcg tggaggagtg caggtggaaa ccatctcccc aggcgacggg cgcaccttcc
      121 ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat
      181 ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga
      241 tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta
      301 tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca
      361 ctctcgtctt cgatgtggag cttctaaaac tggaataata agttgcgaga cgaaaacgaa
      421 ttcaagcttg atatcattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
      481 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
      541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
      661 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      721 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      781 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      841 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      901 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      961 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1021 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1081 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
     1141 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta gaaaaactca
     1201 tcgagcatca agtgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     1261 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     1321 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     1381 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     1441 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     1501 tcatcaaaat cactcgcacc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     1561 cgaaatacgc gatcgccgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     1621 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     1681 tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     1741 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag cctgaccatc
     1801 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     1861 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     1921 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac
     1981 gtttcccgtt gaatatggct cattttagct tccttagctc ctgaaaatct cgataactca
     2041 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     2101 cgatcaagtc aaaagcctcc ggtcggaggc ttttgacttt
//
