LOCUS       sequence-189610-        1954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 22 of EMMA. Functional category: 
            Insulator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1911 HC_Kan_Insulator_FB_p2
FEATURES             Location/Qualifiers
     source          1..1954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      295..326
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        327..429
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             430..1245
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(503..522)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1269..1296
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1308..1895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1388..1407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1917..1946)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt
       61 cgttacgtct cgatggggcc gcgaattctg aaagacccca cctgtaggtt tggcaagccc
      121 agggatgtac gtccctaacc cgctaggggg cagcaactag tcccaggcct gcactgccgc
      181 ctgccggcag gggtccagtc gctagcgcat gcctgcagac tcgagacgaa gacgaattct
      241 ctagatatcg ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa
      301 aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat
      361 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
      421 gaagctaaaa tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc
      481 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
      541 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
      601 aaaggtagcg ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa
      661 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
      721 accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt
      781 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
      841 aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
      901 aacggtttgg ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
      961 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     1021 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1081 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     1141 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     1201 atgaataaat tgcagtttca cttgatgctc gatgagtttt tctaatgagg gcccaaatgt
     1261 aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc
     1321 cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca
     1381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag
     1801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1861 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa
     1921 gaaaggccca cccgtgaagg tgagccagtg agtt
//
