LOCUS       sequence-189612-        2069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 16 of EMMA. Functional category: 
            polyadenylation signal sequence .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2030 HC_Kan_bGHpA_p16
FEATURES             Location/Qualifiers
     source          1..2069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    114..338
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      415..446
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        447..549
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             550..1365
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(623..642)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1389..1416
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1428..2015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1508..1527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2037..2066)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatcaagctt
       61 gaattcgtta cgtctcgccc tctctggggt tcgaaatgac cgaccaagcg acgctgtgcc
      121 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      181 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      241 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      301 caatagcagg catgctgggg atgcggtggg ctctatgggc tccgagacga aacgaattct
      361 ctagatatcg ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa
      421 aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat
      481 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
      541 gaagctaaaa tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc
      601 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
      661 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
      721 aaaggtagcg ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa
      781 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
      841 accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt
      901 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
      961 aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     1021 aacggtttgg ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     1081 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     1141 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1201 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     1261 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     1321 atgaataaat tgcagtttca cttgatgctc gatgagtttt tctaatgagg gcccaaatgt
     1381 aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc
     1441 cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca
     1501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag
     1921 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1981 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa
     2041 gaaaggccca cccgtgaagg tgagccagt
//
