LOCUS       sequence-189613-        3435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning of human PRDX5-K83R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pENTR223-PRDX5K83R
FEATURES             Location/Qualifiers
     source          1..3435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(30..52)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      101..187
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      279..306
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(465..482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(636..1224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(716..735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1317..2108)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2539..2555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2539..2555
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2560..2579
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2560..2578
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2596..2695
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(join(3341..3435,1..5))
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atttggggcc cgagcttaag actggccgtc gttttacaac gtcgtgactg ggaaaacatc
       61 catgctagcg ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
      121 gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      181 gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc
      241 aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc tgacggatgg
      301 cctttttgcg tttctacaaa ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc
      361 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      421 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      481 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      541 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      601 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      661 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      721 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      781 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      841 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      901 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      961 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1021 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1081 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1141 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1201 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1261 cagtggaacg acgcgtaact cacgttaagg gattttggtc atgggtggct cgagggttat
     1321 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     1381 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     1441 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1501 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1561 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1621 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1681 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1741 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1801 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     1861 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     1921 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     1981 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     2041 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     2101 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     2161 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     2221 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2281 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     2341 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     2401 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
     2461 gtctagagga ggcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     2521 gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc actatagggg
     2581 atatcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     2641 caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagt tggcaagagc
     2701 tgtgagatga tattgggtgc caggctgcag gtgaggcctg tgccatctgg ttccacattc
     2761 agggccttca ctatgccatc ctgtaccacc atggagaacc tcttgagacg tcgattccca
     2821 aagatggaca ccagcgaatc atctagtaat aagtctgtct ccttcccaaa ggccccagtg
     2881 ggatcagcca ggagccgaac cttgccttcc gccttgtggg ctcggcccca ctcgccagtc
     2941 acaaaggcat cattaacact cagacaggcc accacctgga ctcccttggc cttcagagcc
     3001 tcagcctgct ccacaaaccc tggcaggtgt gtcttggaac atccaggggt gaaggcccca
     3061 ggaactccaa acagcacacc cttcttgccc ctgaacagct ctgccaggtt caccttgttc
     3121 cctggctccc cttcaaacac ctccactgct gggatggcat ctcccacctt gattggggcc
     3181 atggctgcag cggctctgct gaaactgcgg accccgccag acgcccactc tccttcactg
     3241 caccgtcttg ctgccgctgc cgcagactga ccgccggccc caccgacgag tatatagccc
     3301 gctgagcgtc tcagggcgca cacgccagct agtcccatgg tgccaacttt tttgtacaaa
     3361 gttggcatta taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt
     3421 caaaataaaa tcatt
//
