LOCUS       sequence-189616-        2413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 21 of EMMA. Functional category: 
            Selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2040 HC_Kan_PuroR_p21
FEATURES             Location/Qualifiers
     source          1..2413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             71..667
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     564..584
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     terminator      756..785
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      807..1394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1295..1314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1406..1433)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1457..2272)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2180..2199
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2273..2375)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2376..2407)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac
       61 gtctcgtgga accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
      121 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
      181 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
      241 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
      301 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
      361 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
      421 caaggagccc gcgtggttcc tggccaccgt cggcgtgtcg cccgaccacc agggcaaggg
      481 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
      541 cttcctggaa acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
      601 caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg
      661 tgcctaataa gttgcgagac gaaaacgaat tcaagcttga tatcattcag gacgagcctc
      721 agactccagc gtaactggac tgcaatcaac tcactggctc accttcacgg gtgggccttt
      781 cttcggtaga aaatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      901 actctttttc cgaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      961 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1021 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1081 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1141 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1201 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1261 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1321 ctgtcgggtt tcgccacctc tgacttgagc atcgattttt gtgatgctcg tcaggggggc
     1381 ggagcctatg gaaaaacgcc agcaacgcag aaaggcccac ccgaaggtga gccaggtgat
     1441 tacatttggg ccctcattag aaaaactcat cgagcatcaa gtgaaactgc aatttattca
     1501 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     1561 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     1621 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     1681 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     1741 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcacca accaaaccgt
     1801 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgccgtta aaaggacaat
     1861 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     1921 cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg
     1981 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     2041 attccgtcag ccagtttagc ctgaccatct catctgtaac atcattggca acgctacctt
     2101 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     2161 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     2221 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc attttagctt
     2281 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2341 tatggtgaaa gttggaacct cttacgtgcc gatcaagtca aaagcctccg gtcggaggct
     2401 tttgactttc tgc
//
