LOCUS       sequence-189619-        1881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 6 of EMMA. Functional category: 
            Signal peptide for subcellular targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2058 HC_Kan_Kozak_ATG_Palm_p6
FEATURES             Location/Qualifiers
     source          1..1881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      231..262
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        263..365
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             366..1181
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(439..458)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1205..1232
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1244..1831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1324..1343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(1853..1881,1))
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 cagtgagttg attgcagtcc agttacgctg gagtctgagg ctcgtcctga atgatatcaa
       61 gcttgaattc gttacgtctc gccagccgcc accatgctgt gctgcatgcg ccgcaccaag
      121 caggtggaga agaacgacga cgaccagaag atcggcagcc gagacggacg aattctctag
      181 atatcgctca atactgacca tttaaatcat acctgacctc catagcagaa agtcaaaagc
      241 ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt caccataatg
      301 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
      361 ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca
      421 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
      481 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
      541 gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg acggaattta
      601 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
      661 ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
      721 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
      781 gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
      841 gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
      901 ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt
      961 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     1021 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     1081 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     1141 ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc aaatgtaatc
     1201 acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat aggctccgcc
     1261 cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac ccgacaggac
     1321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa aaagagttgg
     1741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct accgaagaaa
     1861 ggcccacccg tgaaggtgag c
//
