LOCUS       sequence-189622-        1884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 6 of EMMA. Functional category: 
            Signal peptide for subcellular targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2735 HC_Kan_Kozak-ATG-IgkL_p6
FEATURES             Location/Qualifiers
     source          1..1884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     85..144
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     terminator      225..256
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        257..359
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             360..1175
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(433..452)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1199..1226
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1238..1825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1318..1337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1847..1876)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt
       61 cgttacgtct cgccagccgc caccatggaa acagacacac tgctgctatg ggtactgctg
      121 ctctgggttc caggttccac tggtgacagc agccgagacg gacgaattct ctagatatcg
      181 ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga
      241 ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
      301 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
      361 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      421 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      481 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      541 ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc
      601 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      661 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      721 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      781 ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      841 ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      901 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      961 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1021 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     1081 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1141 tgcagtttca cttgatgctc gatgagtttt tctaatgagg gcccaaatgt aatcacctgg
     1201 ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg
     1261 acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1681 cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc
     1741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca
     1861 cccgtgaagg tgagccagtg agtt
//
