LOCUS       sequence-189624-        1864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 8 of EMMA. Functional category: 
            Peptide linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2746 HC_Kan_Linker1_p8
FEATURES             Location/Qualifiers
     source          1..1864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      206..235
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      257..844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     745..764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(856..883)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(907..1722)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1630..1649
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1723..1825)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1826..1857)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 atggaggtca ggtatgattt aaatggtcag tattgagcga tatctagaga attcgtcacg
       61 tctcgaggct ctggactcag atctggcagc ggaggaggca gcgcatctgg cggaagcgga
      121 gcgtcgagac gaaaacgaat tcaagcttga tatcattcag gacgagcctc agactccagc
      181 gtaactggac tgcaatcaac tcactggctc accttcacgg gtgggccttt cttcggtaga
      241 aaatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      361 cgaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      421 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      481 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      541 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      601 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      661 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      721 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      781 tcgccacctc tgacttgagc atcgattttt gtgatgctcg tcaggggggc ggagcctatg
      841 gaaaaacgcc agcaacgcag aaaggcccac ccgaaggtga gccaggtgat tacatttggg
      901 ccctcattag aaaaactcat cgagcatcaa gtgaaactgc aatttattca tatcaggatt
      961 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     1021 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     1081 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     1141 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
     1201 aggccagcca ttacgctcgt catcaaaatc actcgcacca accaaaccgt tattcattcg
     1261 tgattgcgcc tgagcgagac gaaatacgcg atcgccgtta aaaggacaat tacaaacagg
     1321 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     1381 aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca
     1441 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     1501 ccagtttagc ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     1561 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     1621 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     1681 tcgcggcctc gagcaagacg tttcccgttg aatatggctc attttagctt ccttagctcc
     1741 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     1801 gttggaacct cttacgtgcc gatcaagtca aaagcctccg gtcggaggct tttgactttc
     1861 tgct
//
