LOCUS       sequence-189626-        2163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 1 of EMMA. Functional category: 
            Inverted Terminal Repeat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2748 HC_Kan_5'ITR-PB_p1
FEATURES             Location/Qualifiers
     source          1..2163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   103..137
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     terminator      506..537
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        538..640
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             641..1456
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(714..733)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1480..1507
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1519..2106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1599..1618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2128..2157)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa
       61 ttcgttacgt ctcgtaggac tatcctaacg tccacttctt aaccctagaa agatagtctg
      121 cgtaaaattg acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc
      181 gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc
      241 aatgcggtaa gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg
      301 attatctttt acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg
      361 ttctacttac gtgataactt attatatata tattttcttg ttatagatca ggtggatgaa
      421 agcgtgtaat ggcgagacga agacgaattc tctagatatc gctcaatact gaccatttaa
      481 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga
      541 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      601 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga
      661 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      721 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
      781 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
      841 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
      901 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt
      961 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     1021 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt
     1081 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga
     1141 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     1201 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     1261 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     1321 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     1381 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct
     1441 cgatgagttt ttctaatgag ggcccaaatg taatcacctg gctcaccttc gggtgggcct
     1501 ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1561 atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1921 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1981 tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2041 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2101 tctcaagaag atcctttgat tttctaccga agaaaggccc acccgtgaag gtgagccagt
     2161 gag
//
