LOCUS       sequence-189627-        2059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 24 of EMMA. Functional category: 
            Inverted Terminal Repeat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2750 HC_Kan_3'ITR-PB_p24
FEATURES             Location/Qualifiers
     source          1..2059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      403..432
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      454..1041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     942..961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1053..1080)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1104..1919)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1827..1846
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1920..2022)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2023..2054)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctatggaggt caggtatgat ttaaatggtc agtattgagc gatatctaga gaattcgtac
       61 gtctcgcacg tgcatccacg atctagtcac tttgttactt tatagaagaa attttgagtt
      121 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
      181 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacgtcaat
      241 tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag ggttaactac
      301 tcaccctcga gcataacact gcgagacgaa aacgaattca agcttgatat cattcaggac
      361 gagcctcaga ctccagcgta actggactgc aatcaactca ctggctcacc ttcacgggtg
      421 ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      541 gctaccaact ctttttccga ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      961 tatagtcctg tcgggtttcg ccacctctga cttgagcatc gatttttgtg atgctcgtca
     1021 ggggggcgga gcctatggaa aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc
     1081 aggtgattac atttgggccc tcattagaaa aactcatcga gcatcaagtg aaactgcaat
     1141 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     1201 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     1261 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     1321 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     1381 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcaccaacc
     1441 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gccgttaaaa
     1501 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     1561 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt ccctgggatc
     1621 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     1681 ggcataaatt ccgtcagcca gtttagcctg accatctcat ctgtaacatc attggcaacg
     1741 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     1801 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     1861 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcatt
     1921 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1981 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc
     2041 ggaggctttt gactttctg
//
