LOCUS       sequence-18964-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Bruning JC, Michael MD, Winnay JN, Hayashi T, Horsch D, Accili D, 
            Goodyear LJ, Kahn CR
  TITLE     A muscle-specific insulin receptor knockout exhibits features of the
            metabolic syndrome of NIDDM without altering glucose tolerance.
  JOURNAL   Mol Cell. 1998 Nov . 2(5):559-69.
  PUBMED    9844629
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Nov . 2(5):559-69."
COMMENT     Sequence Label: pBS MCK promoter
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    51..172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(62..81)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     116..135
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(221..240)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(222..240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     250..266
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     250..266
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(300..316)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(301..317)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tcgagtcagt gagcgaggaa gcggaagagt ctagagtcga ccagacatga taagatacat
       61 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      121 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttctgcttta
      181 ataagatctg attcgaattc caagcttgga tccgaattcg ccctatagtg agtcgtatta
      241 cgcggccgct ctagaactag tggatccccc gggctgcagg aattcgatat caagcttatc
      301 gataccgtcg acctcgaggc cggcggcctt tgggaccctg tgagcagacc gggttcagac
      361 ttaagaactc agttgctggg ggcttgcagt ccgtcggacc gtgggcttga aaaagggggg
      421 ttctccccct gtgaacctca gccttgcaag gaaacatcag gagcccaagt tgcctgatgt
      481 ggccacatcc tgcctatgtg atcctgctgg ggactcagtc tctctagctg gaaaaggagg
      541 gcaaaacctt ttgtctaaag ccagaaaagg acagtcacat aaacaaaaac aactacaaaa
      601 atacaggatc tggggctggg gttctgctgg cagagtgctt gcccagcaag cttaggaccc
      661 tgggtaacag tttggcgctc agcaaatctg ggtgtggctg ctcatgcctg aaatcccagc
      721 agttcaaggt catcttcatc tacgtagaga gctggagacc agcccgagtc tgggctccta
      781 caca
//
