LOCUS       sequence-189643-        8964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of 
            P39880), with an N-terminal Myc tag and a C-terminal HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Wilson BJ, Harada R, LeDuy L, Hollenberg MD, Nepveu A
  TITLE     CUX1 transcription factor is a downstream effector of the 
            proteinase-activated receptor 2 (PAR2).
  JOURNAL   J Biol Chem. 2009 Jan 2;284(1):36-45. doi: 10.1074/jbc.M803808200. 
            Epub 2008 Oct 24.
  PUBMED    18952606
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M803808200"; journalName: "J Biol Chem"; date: 
            "2009-01-2- 2"; volume: "284"; issue: "1"; pages: "36-45"
COMMENT     Sequence Label: pXJ42-p200 CUX1
FEATURES             Location/Qualifiers
     source          1..8964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        345..548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     499..519
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     545..569
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          801..1373
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1381..1400
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1428..1447
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1428..1446
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1470..1479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1479..1508
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6024..6050
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6084..6205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6121..6140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6175..6194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6239..6256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6410..6998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6490..6509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7169..7960,7961..8029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7792..7811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8030..8134)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8202..8220
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8259..8281)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8381..8400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8467..8922)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8604..8625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8816..8835
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatcggtgcg ggcctcttcg ctattacgcc agctagagtc cgttacataa cttacggtaa
       61 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      121 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      181 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      241 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      301 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      361 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      421 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      481 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      541 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      601 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgg
      661 acccctaggc ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg
      721 gacccttgat tgttctttct ttttcgctat tgtaggccta ggcttttgca aaaagctgga
      781 tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
      841 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
      901 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
      961 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     1021 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     1081 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     1141 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     1201 ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc
     1261 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     1321 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1381 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgtaa tacgactcac
     1441 tatagggcga atagatctgg taccctcgag ccaccatgga gcagaagctc atctctgaag
     1501 aggacctcat gttgtgcgta cgcggagcca ggttgaagag agaactcgat gccaccgcaa
     1561 cggtattggc gaaccggcag gatgaaagtg agcagtccag aaagcggctt atcgaacaga
     1621 gccgggagtt caagaagaac actccagagg atttgcgcaa gcaggtagcg ccgctgctga
     1681 agagtttcca aggagagatt gatgcactga gtaaaagaag caaggaagct gaagcagctt
     1741 tcttgaatgt ctacaaaaga ttgattgacg tcccagatcc cgtaccagct ttggatctcg
     1801 gacagcaact ccagctcaaa gtgcagcgcc tgcacgatat tgaaacagag aaccagaaac
     1861 ttagggaaac tctggaagaa tacaacaagg aatttgctga agtgaaaaat caagaggtta
     1921 cgataaaagc acttaaagag aaaatccgag aatatgaaca gacactgaag aaccaagccg
     1981 aaaccatagc tcttgagaag gaacagaagt tacagaatga ctttgcagaa aaggagagaa
     2041 agctgcagga gacacagatg tccaccacct caaagctgga ggaagctgag cataaggttc
     2101 agagcctaca aacagccctg gaaaaaactc gaacagaatt atttgacctg aaaaccaaat
     2161 acgatgaaga aactactgca aaggccgacg agattgaaat gatcatgacg gaccttgaaa
     2221 gggcaaacca gagggcagag gtggctcaga gagaggcgga gaccttaagg gaacagctct
     2281 catcggccaa tcactccctc cagctggcct cacagatcca gaaggcacca gacgtggagc
     2341 aggccataga ggtgctgacc cgctccagcc tagaagttga gttggccgcc aaggagcggg
     2401 agatcgcaca gctggtggag gacgtgcaga gactccaggc cagcctcacc aagctgcggg
     2461 agaattcggc cagccagatc tcacagcttg agcagcagct gagcgccaaa aacagcacac
     2521 tcaaacaact ggaagaaaaa ctcaaaggcc aggctgacta tgaagaggtg aagaaagagc
     2581 tgaacattct gaagtccatg gagtttgcac cgtccgaggg cgctgggaca caggatgcgg
     2641 ccaagcccct ggaggtgctg ttgctggaga agaaccgctc gctgcagtcc gagaacgccg
     2701 cgctgcgcat ctccaacagc gacctgagcg ggtcagccag gaggaaaggg aaagaccagc
     2761 ctgaaagtcg gcgcccggga tctttgccgg ccccccctcc ttctcagttg ccccgcaacc
     2821 cgggggagca ggcttccaat actaatggta cacaccagtt ctcaccagcg gggttaagtc
     2881 aagacttttt cagctcatcc ctggcaagcc ccagcctacc cctggcttct acaggaaaat
     2941 ttgcactaaa ctctcttctc cagcggcagc taatgcagtc cttctactcc aaggctatgc
     3001 aggaagccgg aagcacaagc atgatttttt caacaggtcc atacagcaca aactccatat
     3061 cttcccaaag tccattacaa caaagcccag atgtcaatgg catggcccca tcccccagcc
     3121 agtcagaaag tgctgggagc gtctccgagg gagaggagat ggacactgca gaaatcgccc
     3181 ggcaggtgaa agagcagctg attaagcaca atatcggaca acgtattttc ggacattatg
     3241 tgttgggact gtctcaaggg tccgtgagcg agattctggc ccggcccaag ccatggaata
     3301 aactgactgt tcgtggcaag gagccatttc acaagatgaa acagttcctc tccgatgagc
     3361 agaacatcct ggccctccgt agcatccaag gcagacaaag agagaatcca ggccagagcc
     3421 tgaacagact atttcaggaa gtaccgaaac gaagaaatgg gtctgaaggt aacatcacca
     3481 cccggatccg agcctcggag actggctctg atgaagccat caagtccatc ctagagcaag
     3541 ccaagaggga gctccaagtg cagaaaactg cagagccggc ccagccttcc tccgcatccg
     3601 gcagcgggaa ctctgatgag cccatccgct ccatcctgca gcaagcccgc cgggagatgg
     3661 aggcccagca ggctgccctc gaccctgcct taaagcaggc accactgtcc cagagtgaca
     3721 tcaccatcct cacccccaag cttctgtcca cctcgcccat gcccaccgtg tccagctacc
     3781 cacctctcgc catctccctg aagaagccct ccgcagctcc tgaggccggt gcctctgctc
     3841 tgccgaaccc cccggccctc aaaaaggagg cccaggacgc ccccgggctg gacccccagg
     3901 gagcagccga ttgtgcacaa ggggtcctga gacaggtgaa aaatgaggtg ggccgcagcg
     3961 gtgcctggaa ggaccactgg tggagcgcgg tgcagccgga gagaagaaat gccgcctcct
     4021 ccgaggaggc caaggccaga gaaacgggcg gcgggaaaga gaagggcagc ggtggcagcg
     4081 gaggtggcag ccagcctcgg gccgagcgca gtcagctcca gggaccctcg tcgtcagagt
     4141 actggaagga gtggcccagc gctgagtccc catactccca gagctcagag ctgagtctga
     4201 ccggggccag ccgcagcgag acaccacaga acagccccct gccatcctcc ccgatcgtgc
     4261 ccatgtccaa gcccaccaag ccctcggtcc ccccgctgac ccccgagcag tacgaggtct
     4321 acatgtacca ggaggtggac accatcgagc tcacccggca ggttaaggaa aagctggcca
     4381 agaacggcat ctgccagaga atcttcgggg agaaggtgct gggcctgtcc cagggcagcg
     4441 tcagcgacat gctgtcccga ccgaagccat ggagcaagct gacgcagaaa ggccgagaac
     4501 ccttcatccg gatgcagctc tggctgaacg gcgagctagg ccagggtgtt ctacccgtcc
     4561 agggccagca gcaagggcca gtcctccact ccgtgacatc gctccaggac ccgctgcagc
     4621 agggctgtgt gagctcagaa agcactccaa agacctccgc cagctgcagc cctgcccctg
     4681 agtccccgat gagttccagt gagtcggtga agagcctgac cgagctggtc cagcagccct
     4741 gtccccccat cgaggcgagc aaggacagca agccaccaga gcccagtgac ccgccagcat
     4801 ccgactccca gcccacaacc ccgctgcctc tctccggaca ctcggccctc agcatccaag
     4861 aattagtagc catgtccccg gagctggaca cctacggcat aaccaagcgg gtgaaggagg
     4921 tgctgacgga caacaacctc ggccagcgct tatttgggga gaccatctta gggctcaccc
     4981 aaggctctgt ctctgacctc cttgcccgcc ccaaaccctg gcataagctc agtctgaaag
     5041 gacgagagcc cttcgtccgg atgcagctgt ggctgaacga ccccaacaat gtggagaagc
     5101 tgatggacat gaaacggatg gagaagaaag cctacatgaa gcggcggcac agctcagtca
     5161 gtgacagcca gccctgcgaa ccgccctctg tcggcaccga gtacagccag ggcgccagcc
     5221 cccagcccca gcaccagctg aagaaacccc gggtggtgct ggctccggag gagaaggagg
     5281 cgctgaaacg agcgtatcag caaaagccat acccgtcacc aaaaaccatc gaagacctcg
     5341 ccacccagct caacctgaaa acaagcaccg tcatcaactg gttccacaac tacaggtctc
     5401 ggatccgcag agaactgttc attgaggaaa ttcaggccgg gagtcagggc caggcgggcg
     5461 ccagcgactc accctcggcc cgcagcggcc gggcggcgcc cagctcggag ggcgacagct
     5521 gcgacggcgt ggaggccact gagggcccag gcagcgccga caccgaggag cccaagtctc
     5581 agggagaggc cgagcgggag gaggtgccgc ggccggcgga gcagacggag ccgccgccct
     5641 cggggacccc gggcccggac gacgcccgcg acgacgacca cgagggaggc cccgtggaag
     5701 gcccggggcc cctgcccagc cccgcctccg cgaccgccac cgccgcgccc gcggcccccg
     5761 aggacgccgc tacctcagcc gccgccgcgc cgggggaggg ccccgcggcc ccgacgtccg
     5821 cgccaccgcc cagcaacagc agcagcagca gcgccccccg caggcccagc tcgctgcaga
     5881 gccttttcgg cctccccgag gccgcgggcg cccgggactc gcgcgacaac cccctgcgca
     5941 agaagaaggc cgcgaacttg aacagcatca tccaccgcct ggagaaggcc gccagccggg
     6001 aggaacctat cgaatgggag ttctatccat acgacgtacc tgactacgca tgagcggccg
     6061 cgaattcgat cttattaaag cagaacttgt ttattgcagc ttataatggt tacaaataaa
     6121 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6181 tgtccaaact catcaatgta tcttatcatg tctggtcgac tctagactct tccgcttcct
     6241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6601 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6841 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttctttt
     8041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8161 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8221 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8281 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     8341 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     8401 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8461 atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     8521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat tcgccattca
     8941 ggctacgcaa ctgttgggaa gggc
//
