LOCUS       sequence-189646-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human PRDX5-K83R in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pLD-puro-Cc-PRDX5K83R-VA
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     246..266
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             477..710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    782..903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(819..838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     873..892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      943..1078
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1098..1117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1099..1117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1127..1144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1127..1143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1136..1158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1285..1740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1372..1391)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1582..1603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1766..1870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1871..1939,1940..2731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2089..2108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2902..3490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3391..3410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3644..3661
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3778..3799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3814..3819,3820..3837,3838..3844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3852..3868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3857..3879
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3876..3892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3876..3892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3911..3931
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3913..3931
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3959..4185
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4186..4366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4413..4538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5031..5264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5449..5493
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     5791..5811
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        6100..6303
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6254..6274
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6300..6324
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6355..6379
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7025..7049)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7129..7152
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7189..7212
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7225..7242
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7252..7272
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7285..7305
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7318..7383
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7431..7548
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7597..8107
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8026..8044
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(8129..8379,1..349)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8129..8148)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
       61 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      121 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      181 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      241 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      301 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      361 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcggtac ctttaagacc
      421 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
      481 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
      541 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      601 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      661 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
      721 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
      781 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      841 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      901 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
      961 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1021 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1081 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     1141 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1201 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1261 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1321 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1381 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1441 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1501 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     1561 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     1621 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1681 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     1741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1861 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1921 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1981 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2041 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2101 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2161 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2221 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2281 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2341 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2401 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2461 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2521 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2581 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2641 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2701 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2761 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2821 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3541 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3601 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3661 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3721 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3781 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     3841 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     3901 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     3961 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     4021 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     4081 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     4141 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     4201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4381 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4441 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4501 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4561 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4621 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4681 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4741 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4801 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4861 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     4921 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     4981 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5041 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5101 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5161 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5221 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5281 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5341 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5401 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5461 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5521 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5581 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5641 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     5701 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga
     5761 gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat
     5821 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
     5881 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     5941 ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat tgacgtcaat
     6001 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     6061 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     6121 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     6181 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     6241 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     6301 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac gcgtacaagt
     6361 ttgtacaaaa aagttggcac catgggacta gctggcgtgt gcgccctgag acgctcagcg
     6421 ggctatatac tcgtcggtgg ggccggcggt cagtctgcgg cagcggcagc aagacggtgc
     6481 agtgaaggag agtgggcgtc tggcggggtc cgcagtttca gcagagccgc tgcagccatg
     6541 gccccaatca aggtgggaga tgccatccca gcagtggagg tgtttgaagg ggagccaggg
     6601 aacaaggtga acctggcaga gctgttcagg ggcaagaagg gtgtgctgtt tggagttcct
     6661 ggggccttca cccctggatg ttccaagaca cacctgccag ggtttgtgga gcaggctgag
     6721 gctctgaagg ccaagggagt ccaggtggtg gcctgtctga gtgttaatga tgcctttgtg
     6781 actggcgagt ggggccgagc ccacaaggcg gaaggcaagg ttcggctcct ggctgatccc
     6841 actggggcct ttgggaagga gacagactta ttactagatg attcgctggt gtccatcttt
     6901 gggaatcgac gtctcaagag gttctccatg gtggtacagg atggcatagt gaaggccctg
     6961 aatgtggaac cagatggcac aggcctcacc tgcagcctgg cacccaatat catctcacag
     7021 ctcttgccaa ctttcttgta caaagtggtt gattctagag cgatcgccag agagctgttc
     7081 aacctgctgg gcgagaacca gccccccgtg gtgatcaagg cgatcgcctg gagccaccct
     7141 cagttcgaga agggaggagg aagcggcgga ggcagcggag gaggaagctg gagccacccg
     7201 cagttcgaga aaggcggaag cggccatcac catcaccatc accctgcagg agaaaatctg
     7261 tacttccagg ggagaggagg cagcgagaac ctgtactttc aggggcctgc aggaagagac
     7321 tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac
     7381 aagtagttcg aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
     7441 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     7501 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     7561 agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca
     7621 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     7681 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     7741 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     7801 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     7861 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     7921 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     7981 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     8041 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     8101 tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     8161 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     8221 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     8281 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     8341 cgcggtggcg gtctggacca cgccggagag cgtcgaagc
//
