LOCUS       sequence-189648-        6351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of 
            P39880), with an N-terminal Myc tag and a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Truscott M, Harada R, Vadnais C, Robert F, Nepveu A
  TITLE     p110 CUX1 cooperates with E2F transcription factors in the 
            transcriptional activation of cell cycle-regulated genes.
  JOURNAL   Mol Cell Biol. 2008 May;28(10):3127-38. doi: 10.1128/MCB.02089-07. 
            Epub 2008 Mar 17.
  PUBMED    18347061
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.02089-07"; journalName: "Mol Cell Biol"; date: 
            "2008-05"; volume: "28"; issue: "10"; pages: "3127-38"
COMMENT     Sequence Label: pXJ42-p110 CUX1
FEATURES             Location/Qualifiers
     source          1..6351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        345..548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     499..519
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     545..569
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          801..1373
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1381..1400
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1428..1447
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1428..1446
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1470..1479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1479..1508
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3411..3437
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3471..3592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3508..3527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3562..3581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3626..3643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3797..4385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3877..3896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4556..5347,5348..5416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5179..5198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5417..5521)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5589..5607
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5646..5668)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5768..5787
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5854..6309)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5991..6012)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6203..6222
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatcggtgcg ggcctcttcg ctattacgcc agctagagtc cgttacataa cttacggtaa
       61 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      121 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      181 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      241 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      301 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      361 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      421 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      481 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      541 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      601 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgg
      661 acccctaggc ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg
      721 gacccttgat tgttctttct ttttcgctat tgtaggccta ggcttttgca aaaagctgga
      781 tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
      841 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
      901 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
      961 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     1021 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     1081 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     1141 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     1201 ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc
     1261 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     1321 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1381 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgtaa tacgactcac
     1441 tatagggcga atagatctgg taccctcgag ccaccatgga gcagaagctc atctctgaag
     1501 aggacctcac gcgtggtcga cagaattact ggaaggagtg gcccagcgct gagtccccat
     1561 actcccagag ctcagagctg agtctgaccg gggccagccg cagcgagaca ccacagaaca
     1621 gccccctgcc atcctccccg atcgtgccca tgtccaagcc caccaagccc tcggtccccc
     1681 cgctgacccc cgagcagtac gaggtctaca tgtaccagga ggtggacacc atcgagctca
     1741 cccggcaggt taaggaaaag ctggccaaga acggcatctg ccagagaatc ttcggggaga
     1801 aggtgctggg cctgtcccag ggcagcgtca gcgacatgct gtcccgaccg aagccatgga
     1861 gcaagctgac gcagaaaggc cgagaaccct tcatccggat gcagctctgg ctgaacggcg
     1921 agctaggcca gggtgttcta cccgtccagg gccagcagca agggccagtc ctccactccg
     1981 tgacatcgct ccaggacccg ctgcagcagg gctgtgtgag ctcagaaagc actccaaaga
     2041 cctccgccag ctgcagccct gcccctgagt ccccgatgag ttccagtgag tcggtgaaga
     2101 gcctgaccga gctggtccag cagccctgtc cccccatcga ggcgagcaag gacagcaagc
     2161 caccagagcc cagtgacccg ccagcatccg actcccagcc cacaaccccg ctgcctctct
     2221 ccggacactc ggccctcagc atccaagaat tagtagccat gtccccggag ctggacacct
     2281 acggcataac caagcgggtg aaggaggtgc tgacggacaa caacctcggc cagcgcttat
     2341 ttggggagac catcttaggg ctcacccaag gctctgtctc tgacctcctt gcccgcccca
     2401 aaccctggca taagctcagt ctgaaaggac gagagccctt cgtccggatg cagctgtggc
     2461 tgaacgaccc caacaatgtg gagaagctga tggacatgaa acggatggag aagaaagcct
     2521 acatgaagcg gcggcacagc tcagtcagtg acagccagcc ctgcgaaccg ccctctgtcg
     2581 gcaccgagta cagccagggc gccagccccc agccccagca ccagctgaag aaaccccggg
     2641 tggtgctggc tccggaggag aaggaggcgc tgaaacgagc gtatcagcaa aagccatacc
     2701 cgtcaccaaa aaccatcgaa gacctcgcca cccagctcaa cctgaaaaca agcaccgtca
     2761 tcaactggtt ccacaactac aggtctcgga tccgcagaga actgttcatt gaggaaattc
     2821 aggccgggag tcagggccag gcgggcgcca gcgactcacc ctcggcccgc agcggccggg
     2881 cggcgcccag ctcggagggc gacagctgcg acggcgtgga ggccactgag ggcccaggca
     2941 gcgccgacac cgaggagccc aagtctcagg gagaggccga gcgggaggag gtgccgcggc
     3001 cggcggagca gacggagccg ccgccctcgg ggaccccggg cccggacgac gcccgcgacg
     3061 acgaccacga gggaggcccc gtggaaggcc cggggcccct gcccagcccc gcctccgcga
     3121 ccgccaccgc cgcgcccgcg gcccccgagg acgccgctac ctcagccgcc gccgcgccgg
     3181 gggagggccc cgcggccccg acgtccgcgc caccgcccag caacagcagc agcagcagcg
     3241 ccccccgcag gcccagctcg ctgcagagcc ttttcggcct ccccgaggcc gcgggcgccc
     3301 gggactcgcg cgacaacccc ctgcgcaaga agaaggccgc gaacttgaac agcatcatcc
     3361 accgcctgga gaaggccgcc agccgggagg aacctatcga atgggagttc tatccatacg
     3421 acgtacctga ctacgcatga gcggccgcga attcgatctt attaaagcag aacttgttta
     3481 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3541 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3601 ggtcgactct agactcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3961 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     4021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5401 aatgttgaat actcatactc ttcttttttc aatattattg aagcatttat cagggttatt
     5461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5521 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     5581 cctataaaaa taggcgtatc acgaggcccc tttcgtctcg cgcgtttcgg tgatgacggt
     5641 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     5701 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     5761 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     5821 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa
     5881 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5941 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6001 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6061 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6121 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6181 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6241 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6301 agggcgcgtc gcgccattcg ccattcaggc tacgcaactg ttgggaaggg c
//
