LOCUS       sequence-18965-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Bruning JC, Michael MD, Winnay JN, Hayashi T, Horsch D, Accili D, 
            Goodyear LJ, Kahn CR
  TITLE     A muscle-specific insulin receptor knockout exhibits features of the
            metabolic syndrome of NIDDM without altering glucose tolerance.
  JOURNAL   Mol Cell. 1998 Nov . 2(5):559-69.
  PUBMED    9844629
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Nov . 2(5):559-69."
COMMENT     Sequence Label: pBS MCK promoter
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    79..200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(90..109)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     144..163
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(249..268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(250..268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     278..294
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     278..294
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(328..344)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..345)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 aaaagctgga gctccaccgc ggtggagctc gagtcagtga gcgaggaagc ggaagagtct
       61 agagtcgacc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      121 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      181 taagctgcaa taaacaagtt ctgctttaat aagatctgat tcgaattcca agcttggatc
      241 cgaattcgcc ctatagtgag tcgtattacg cggccgctct agaactagtg gatcccccgg
      301 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgaggccg gcggcctttg
      361 ggaccctgtg agcagaccgg gttcagactt aagaactcag ttgctggggg cttgcagtcc
      421 gtcggaccgt gggcttgaaa aaggggggtt ctccccctgt gaacctcagc cttgcaagga
      481 aacatcagga gcccaagttg cctgatgtgg ccacatcctg cctatgtgat cctgctgggg
      541 actcagtctc tctagctgga aaaggagggc aaaacctttt gtctaaagcc agaaaaggac
      601 agtcacataa acaaaaacaa ctacaaaaat acaggatctg gggctggggt tctgctggca
      661 gagtgcttgc ccagcaagct t
//
