LOCUS       sequence-189653-        9456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505
            of P39880), with an N-terminal Myc tag and a C-terminal HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Vadnais C, Awan AA, Harada R, Clermont PL, Leduy L, Berube G, Nepveu
            A
  TITLE     Long-range transcriptional regulation by the p110 CUX1 homeodomain 
            protein on the ENCODE array.
  JOURNAL   BMC Genomics. 2013 Apr 16;14:258. doi: 10.1186/1471-2164-14-258.
  PUBMED    23590133
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-04-16"; volume: "14:258. doi"; pages: " 
            10.1186/1471-2164-14-258"
COMMENT     Sequence Label: pLenti6/V5-DEST-p110 CUX1
FEATURES             Location/Qualifiers
     source          1..9456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2274..2294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2320..2344
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2440..2464
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2528..2557
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4835..4861
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4888..4912)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4965..5006
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5043..5063)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5048..5378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5229..5364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5426..5473
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5492..5890
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5977..6210
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6282..6403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6319..6338)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6373..6392
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6443..6578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6598..6617)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6599..6617)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6627..6644)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6627..6643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6636..6658)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6785..7240
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6872..6891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7082..7103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7266..7370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7371..7439,7440..8231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7589..7608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8402..8990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8891..8910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9144..9161
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9278..9299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9314..9319,9320..9337,9338..9344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9352..9368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9357..9379
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9376..9392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9376..9392
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9411..9431
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9413..9431
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2461 ggcttcgaag gagatagaac caattctcta aggaaatact taaccatggt cgactggatc
     2521 caccatggag cagaagctca tctctgaaga ggacctctcc acctcgccca tgcccaccgt
     2581 gtccagctac ccacctctcg ccatctccct gaagaagccc tccgcagctc ctgaggccgg
     2641 tgcctctgct ctgccgaacc ccccggccct caaaaaggag gcccaggacg cccccgggct
     2701 ggacccccag ggagcagccg attgtgcaca aggggtcctg agacaggtga aaaatgaggt
     2761 gggccgcagc ggtgcctgga aggaccactg gtggagcgcg gtgcagccgg agagaagaaa
     2821 tgccgcctcc tccgaggagg ccaaggccag agaaacgggc ggcgggaaag agaagggcag
     2881 cggtggcagc ggaggtggca gccagcctcg ggccgagcgc agtcagctcc agggaccctc
     2941 gtcgtcagag tactggaagg agtggcccag cgctgagtcc ccatactccc agagctcaga
     3001 gctgagtctg accggggcca gccgcagcga gacaccacag aacagccccc tgccatcctc
     3061 cccgatcgtg cccatgtcca agcccaccaa gccctcggtc cccccgctga cccccgagca
     3121 gtacgaggtc tacatgtacc aggaggtgga caccatcgag ctcacccggc aggttaagga
     3181 aaagctggcc aagaacggca tctgccagag aatcttcggg gagaaggtgc tgggcctgtc
     3241 ccagggcagc gtcagcgaca tgctgtcccg accgaagcca tggagcaagc tgacgcagaa
     3301 aggccgagaa cccttcatcc ggatgcagct ctggctgaac ggcgagctag gccagggtgt
     3361 tctacccgtc cagggccagc agcaagggcc agtcctccac tccgtgacat cgctccagga
     3421 cccgctgcag cagggctgtg tgagctcaga aagcactcca aagacctccg ccagctgcag
     3481 ccctgcccct gagtccccga tgagttccag tgagtcggtg aagagcctga ccgagctggt
     3541 ccagcagccc tgtcccccca tcgaggcgag caaggacagc aagccaccag agcccagtga
     3601 cccgccagca tccgactccc agcccacaac cccgctgcct ctctccggac actcggccct
     3661 cagcatccaa gaattagtag ccatgtcccc ggagctggac acctacggca taaccaagcg
     3721 ggtgaaggag gtgctgacgg acaacaacct cggccagcgc ttatttgggg agaccatctt
     3781 agggctcacc caaggctctg tctctgacct ccttgcccgc cccaaaccct ggcataagct
     3841 cagtctgaaa ggacgagagc ccttcgtccg gatgcagctg tggctgaacg accccaacaa
     3901 tgtggagaag ctgatggaca tgaaacggat ggagaagaaa gcctacatga agcggcggca
     3961 cagctcagtc agtgacagcc agccctgcga accgccctct gtcggcaccg agtacagcca
     4021 gggcgccagc ccccagcccc agcaccagct gaagaaaccc cgggtggtgc tggctccgga
     4081 ggagaaggag gcgctgaaac gagcgtatca gcaaaagcca tacccgtcac caaaaaccat
     4141 cgaagacctc gccacccagc tcaacctgaa aacaagcacc gtcatcaact ggttccacaa
     4201 ctacaggtct cggatccgca gagaactgtt cattgaggaa attcaggccg ggagtcaggg
     4261 ccaggcgggc gccagcgact caccctcggc ccgcagcggc cgggcggcgc ccagctcgga
     4321 gggcgacagc tgcgacggcg tggaggccac tgagggccca ggcagcgccg acaccgagga
     4381 gcccaagtct cagggagagg ccgagcggga ggaggtgccg cggccggcgg agcagacgga
     4441 gccgccgccc tcggggaccc cgggcccgga cgacgcccgc gacgacgacc acgagggagg
     4501 ccccgtggaa ggcccggggc ccctgcccag ccccgcctcc gcgaccgcca ccgccgcgcc
     4561 cgcggccccc gaggacgccg ctacctcagc cgccgccgcg ccgggggagg gccccgcggc
     4621 cccgacgtcc gcgccaccgc ccagcaacag cagcagcagc agcgcccccc gcaggcccag
     4681 ctcgctgcag agccttttcg gcctccccga ggccgcgggc gcccgggact cgcgcgacaa
     4741 ccccctgcgc aagaagaagg ccgcgaactt gaacagcatc atccaccgcc tggagaaggc
     4801 cgccagccgg gaggaaccta tcgaatggga gttctatcca tacgacgtac ctgactacgc
     4861 atgagcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtg gttgatatcc
     4921 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     4981 accctctcct cggtctcgat tctacgcgta ccggttagta atgagtttgg aattaattct
     5041 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     5101 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5161 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5221 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5281 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5341 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5401 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     5461 agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc
     5521 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     5581 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     5641 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     5701 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     5761 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     5821 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     5881 ggagggctaa gcacaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     5941 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6001 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6061 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6121 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6181 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6241 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6301 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6361 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6421 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6481 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6541 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     6601 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     6661 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6721 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6781 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6901 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6961 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7021 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7081 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7141 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7201 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7261 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7321 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7381 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7441 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7501 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7561 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7621 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7681 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7741 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7801 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7861 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7921 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7981 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8041 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8101 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8161 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8221 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8281 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8341 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8401 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8461 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8521 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8581 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8641 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8701 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8761 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8821 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8881 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8941 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9001 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9061 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9121 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9181 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9241 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9301 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9361 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     9421 ctcactaaag ggaacaaaag ctggagctgc aagctt
//
