LOCUS       sequence-189667-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PI(WT): Photo inhibitable (blue light sensitive).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, 
            Dokholyan NV, Hahn KM
  TITLE     Engineering extrinsic disorder to control protein activity in living
            cells.
  JOURNAL   Science. 2016 Dec 16;354(6318):1441-1444.
  PUBMED    27980211
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-12-16"; volume: "354(6318)"; pages: "1441-1444"
COMMENT     Sequence Label: pBabe-tetoff-mVenus-PICdc42
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..65)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     439..462
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    530..546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(560..592)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
ORIGIN
        1 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
       61 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
      121 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
      181 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
      241 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
      301 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
      361 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
      421 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
      481 aagcgcctcc cctacccggt agaattgacc tgcaggggcc ctcgacaaat tgttatccgc
      541 tcacaattcc acactcgaga tcctcggggt cttccggggc gagttctggc tggcttgcac
      601 gtgtatacag ctgtgcggcc gcaagctttt atagcagcac acacctgcgg ctcttcttcg
      661 gttctggagg ctccagggca gccaatattg cttcgtcaaa tacattcttt aggcctttct
      721 gtgtaagtgc agaacactcc acatacttga cagccttcag gtcacggg
//
