LOCUS       sequence-18967-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Hamburger AE, West AP, Bjorkman PJ
  TITLE     Crystal structure of a polymeric immunoglobulin binding fragment of 
            the human polymeric immunoglobulin receptor.
  JOURNAL   Structure. 2004 Nov . 12(11):1925-35.
  PUBMED    15530357
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2004 Nov . 12(11):1925-35."
COMMENT     Sequence Label: pET20b-pIgR D1-H6
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             363..380
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(433..451)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      447..494
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(618..637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtttacttta agaaggagat atacatatga agagtcccat atttggtccc gaggaggtga
       61 atagtgtgga aggtaactca gtgtccatca cgtgctacta cccacccacc tctgtcaacc
      121 ggcacacccg gaagtactgg tgccggcagg gagctagagg tggctgcata accctcatct
      181 cctcggaggg ctacgtctcc agcaaatatg caggcagggc taacctcacc aacttcccgg
      241 agaacggcac atttgtggtg aacattgccc agctgagcca ggatgactcc gggcgctaca
      301 agtgtggcct gggcatcaat agccgaggcc tgtcctttga tgtcagcctg gaggtcctcg
      361 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      421 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      481 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      541 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      601 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      661 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      721 gcacctcg
//
