LOCUS       sequence-18968-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Da Costa LT, Jen J, He TC, Chan TA, Kinzler KW, Vogelstein B
  TITLE     Converting cancer genes into killer genes.
  JOURNAL   Proc Natl Acad Sci U S A. 1996 Apr 30. 93(9):4192-6.
  PUBMED    8633039
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1996 Apr 30. 93(9):4192-6."
COMMENT     Sequence Label: pGRE-GFP
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..182
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        complement(216..234)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(217..234)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(270..289)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    343..391
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(421..438)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(421..437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(430..452)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(646..665)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgcccttgc tcaccatccg cggggattca gcggccgcgg gtacaacctc gagtaccgga
       61 tccattatat accctctaga gtctccgctc ggaggacagt actccgctcg gaggacagta
      121 ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgctcggag gacagtactc
      181 cgacctgcag gcatgcaagc ttcagctgct cgagttctat agtgtcacct aaataagccg
      241 aattcgttct ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt
      301 tcgttttgtt ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac
      361 acagatgtaa tgaaaataaa gatattttat tgcggccgct ccaagtacct cccggaattc
      421 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      481 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      541 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      601 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      661 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      721 ttgtctgctc ccggcatccg cttacagacn agctgtgacc gtctccggag ctgcatgtgt
      781 caaagtttca ccgtcatcac gagccg
//
