LOCUS       sequence-189687-        2210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoterless iLOV gene for recombineering, flanked by BsmbI 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2210)
  TITLE     iLOV Recombineering Target Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSAH031
FEATURES             Location/Qualifiers
     source          1..2210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     702..721
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     786..805
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      913..1501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1402..1421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1655..1672
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2142..2164
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(2189..2210,1..794))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(2189..2210,1..794))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
       61 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      121 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      181 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      241 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      301 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag gcgaaatacg
      361 cgatcactgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      421 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      481 gttttgccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      541 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      601 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
      661 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
      721 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
      781 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
      841 catgaccatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
      901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1561 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1621 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1681 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcacgtc
     1741 tctgtccatc gaaaaaaact tcgttatcac cgacccgcgc ttacctgaca atccaatcat
     1801 ctttgcctca gatggttttc tcgagctaac cgaatacagc cgtgaagaaa ttctgggtcg
     1861 taatgctcgt ttcctgcagg gcccggaaac cgaccaagct acggttcaga aaattcgcga
     1921 tgcaattcgc gatcagcgtg agactaccgt tcaattgatc aactacacca aaagcggtaa
     1981 aaaattttgg aacctgttgc atcttcaacc ggtacgcgac caaaaaggcg agcttcagta
     2041 ctttatcggc gtgcagctgg atgggagtga ccatgtgagc gagagacgct gacgggcttg
     2101 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc accgggagct gcatgtgtca
     2161 gaggttttca ccgtcatcac cgaaacgctt agaaaaactc atcgagcatc
//
