LOCUS       sequence-18969-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Jiang W, Zhou L, Breyer B, Feng T, Cheng H, Haydon R, Ishikawa A, He
            TC
  TITLE     Tetracycline-regulated gene expression mediated by a novel chimeric 
            repressor that recruits histone deacetylases in mammalian cells.
  JOURNAL   J Biol Chem. 2001 Nov 30. 276(48):45168-74.
  PUBMED    11581265
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Nov 30. 276(48):45168-74."
COMMENT     Sequence Label: pTHE
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    103..121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(186..389)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(215..235)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 accgcggacc ggtcgacagg cctgaattcg gtgtcttcta tggaggtcaa aacagcgtgg
       61 atggcgtctc caggcgatct gacggttcac taaacagctc gatctctatc actgataggg
      121 agatctctat cactgatagg gagatctcta tcactgatag ggagatctct atcactgata
      181 gggagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      241 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      301 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
      361 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
      421 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
      481 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
      541 aagtgggcag tttaccgtat atactccacc cattgacgtc aatggaaagt ccctattggc
      601 gttactatgg gaacatacgt cattattgac gtcaatgggc ggtggtcgtt gggcggtcag
      661 ccaggcgggc catt
//
