LOCUS       sequence-18970-e         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Park JY, Luo Q, Jiang W, Kang Q, Peng Y, Strom C, Luu HH, Haydon RC,
            He TC
  TITLE     Dual regulation of gene expression mediated by tetracycline and Cre 
            recombinase.
  JOURNAL   Biotechniques. 2004 Mar . 36(3):390-2, 394, 396.
  PUBMED    15038152
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2004 Mar . 36(3):390-2, 394, 396."
COMMENT     Sequence Label: pTLOX
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(78..111)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    236..254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(256..355)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(285..305)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagagcttgg ctggacgtaa
       61 actcctcttc agacctaata acttcgtata gcatacatta tacgaagtta tattaagggt
      121 tattgaatat gatcggaatt cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc
      181 tccaggcgat ctgacggttc actaaacagc tcgatctcta tcactgatag ggagatctct
      241 atcactgata gggagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      301 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      361 aaactaactc ccattgacgt caatggggtg gagacttgga tatccccgta agtcaaaccg
      421 ctatccgcgc ccattgatgt actgccgaaa c
//
