LOCUS       sequence-18971-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Feng T, Li Z, Jiang W, Breyer B, Zhou L, Cheng H, Haydon RC, 
            Ishikawa A, Joudeh MA, He TC
  TITLE     Increased efficiency of cloning large DNA fragments using a lower 
            copy number plasmid.
  JOURNAL   Biotechniques. 2002 May . 32(5):992, 994, 996 passim.
  PUBMED    12019795
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2002 May . 32(5):992, 994, 996 passim."
COMMENT     Sequence Label: pMOLUC
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(498..517)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(499..517)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(524..540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(607..624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attacccctc actaaaggga acaaagctgg tacgaggaca ggctggagcc atgggcatgg
       61 ctactcaagc tgatttgatg gagttggaca tggccatggc tggtgaccac gtcgtggaat
      121 gccttcgaat tcagcacctg cacatgggac gtcgacctga ggtaattata acccgggccc
      181 tatatatgga tccaattgca atgatcatca tgacagatct gcgcgcgatc gatatcagcg
      241 ctttaaattt gcgcatgcta gctatagttc tagaggtacc ggttgttaac gttagccggc
      301 tacgtatact ccggaatatt aataggccta ggatgcatat ggcggccgcc tgcagctggc
      361 gccatcgata cgcgtacgtc gcgaccgcgg acatgtacag agctcgagaa gtactagtgg
      421 ccacgtgggc cgtgcacctt aagcttttaa ataaggagga ataacatatg accatgatta
      481 cgccaagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
      541 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
      601 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctg
//
