LOCUS       sequence-189716-        5634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF268 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF268
FEATURES             Location/Qualifiers
     source          1..5634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2945..3044)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3061..3080)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3062..3080)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3085..3101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3085..3101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3532..4323
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4416..5004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4905..4924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5158..5175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5334..5361)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5453..5539)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5588..5610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5602..5619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5603..5619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatggccac cagggtccgg
      121 acagcttcta tttgggtccc acctctccaa gaacgaaaca gttcatggga taggatcaga
      181 aagctccaag gtcaggaatc catcttgggc caagggactc ctggtctgca acctctccct
      241 ggaacaccca ggcagaagca gaagagtcgc agaatagaga aagtcctaga gtggctgttt
      301 atttcccaag agcagccaaa aatcaccaag tcctggggac ctttgtcatt catggatgtg
      361 tttgtggatt ttacctggga ggagtggcag ctgctagacc cagcacagaa gtgcctgtac
      421 aggagtgtga tgttggagaa ctatagcaac ctggtgtccc tagggtacca acacaccaaa
      481 cctgatatca tcttcaagtt ggaacaagga gaagagctgt gtatggtgca ggcccaagtt
      541 ccaaatcaga cctgtccaaa cacagtctgg aaaattgatg atcttatgga ttggcatcag
      601 gaaaataaag acaagctggg aagtacggca aaaagctttg aatgcactac atttggaaaa
      661 ctatgtcttc ttagtacaaa gtatctttca agacaaaaac ctcataaatg tggcacgcat
      721 ggaaagagtt tgaaatatat agatttcact agtgattatg ctagaaataa tcctaatggg
      781 tttcaggtac atggaaaatc attcttccat tctaaacatg agcaaactgt tattggaata
      841 aaatactgtg aaagtattga atctggaaaa accgtcaata agaaatcgca acttatgtgc
      901 caacaaatgt atatgggcga aaaacccttt ggatgcagct gttgtgagaa agccttcagc
      961 agcaagtcat accttctagt gcatcagcaa actcatgccg aagagaaacc ctatggttgt
     1021 aatgaatgtg ggaaagactt cagtagtaaa tcatacctca ttgtacatca gagaattcat
     1081 acaggagaga aactacatga atgcagtgaa tgcaggaaaa cattcagttt ccattcacag
     1141 cttgttatac atcagagaat tcacacaggt gagaatccct atgagtgctg tgaatgtggg
     1201 aaagtcttca gtaggaaaga ccagcttgtt tcacaccaga aaactcattc aggacagaaa
     1261 ccatatgtgt gtaatgaatg tgggaaagct tttggtttaa aatcacagct cattatacat
     1321 gaaagaattc atacaggaga gaaaccatat gaatgcaatg aatgtcagaa agcctttaat
     1381 acaaagtcaa accttatggt acatcagaga acccatacag gggagaaacc ttatgtttgt
     1441 agtgattgtg gaaaagcctt tacattcaag tcacagctca ttgtacatca ggggattcac
     1501 acaggagtaa agccctatgg gtgtattcag tgtggtaaag gattcagttt gaaatcacag
     1561 ctcattgtac atcagagaag tcacacagga atgaaacctt atgtatgcaa tgaatgtggc
     1621 aaagccttca ggagcaagtc ataccttatt atacatacaa ggactcatac aggagaaaaa
     1681 ctccatgaat gcaacaattg tgggaaagcc ttcagtttta aatcacagct cattatacat
     1741 cagaggattc atacaggaga gaacccctat gaatgccatg aatgtgggaa agccttcagt
     1801 cggaaatacc agcttatttc acaccagaga actcatgcag gagagaagcc ttatgaatgc
     1861 accgactgtg gaaaggcttt tggtttaaag tcacagctta ttatacacca gagaactcat
     1921 acaggggaga aaccatttga atgtagtgag tgtcagaaag cctttaatac aaagtcaaac
     1981 ctgattgtac atcagagaac tcatacagga gagaaaccct atagttgtaa tgaatgtgga
     2041 aaagccttta cgttcaaatc acagctcatt gtacataaag gagtgcacac tggagtaaaa
     2101 ccctatggat gcagtcaatg tgcaaaaacc tttagtttta agtcccagct cattgtacat
     2161 cagagaagtc acacaggagt aaaaccatat ggatgcagtg agtgtgggaa agccttcagg
     2221 agcaagtcat accttattat acatatgaga actcatacag gagagaaacc acatgagtgc
     2281 agggaatgcg ggaaatcctt tagtttcaat tcacaactca ttgtgcatca gagaattcac
     2341 acaggagaaa atccctatga atgcagtgaa tgtgggaaag cctttaatag gaaagaccag
     2401 ctcatttcac atcagcgaac tcatgcaggg gaaaagcctt atgggtgcag tgaatgtggg
     2461 aaagctttta gcagcaagtc atacctaatt atacacatga gaactcattc aggtgaaaaa
     2521 ccatatgaat gtaatgaatg tgggaaagcc ttcatttgga aatcactact cattgtacat
     2581 gagcgaactc atgcaggggt caacccttat aaatgcagtc aatgtgagaa atccttcagt
     2641 gggaaattac gccttcttgt acaccagaga atgcacacaa gagagaaacc atatgaatgc
     2701 agtgagtgtg gaaaagcctt cattaggaat tctcaactca ttgtacatca aagaactcat
     2761 tcaggagaga aaccctatgg gtgcaatgaa tgtgggaaaa ccttctctca aaaatcaatt
     2821 ctcagtgcac atcagagaac acatacagga gagaagcctt gtaagtgcac tgaatgtggg
     2881 aaagcctttt gttggaagtc acagctcatt atgcatcaga gaactcatgt agatgacaaa
     2941 catttgccaa ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     3001 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     3061 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     3121 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc
     3181 agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg
     3241 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     3301 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     3361 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     3421 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     3481 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa
     3541 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     3601 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     3661 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     3721 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     3781 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     3841 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     3901 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     3961 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     4021 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     4081 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     4141 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     4201 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     4261 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     4321 taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     4381 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4441 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4501 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4561 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4621 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4681 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4741 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4801 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4861 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4921 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4981 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5041 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5101 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5161 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5281 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     5341 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     5401 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     5461 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     5521 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     5581 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
