LOCUS       sequence-18974-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Luu HH, Zhou L, Haydon RC, Deyrup AT, Montag AG, Huo D, Heck R, 
            Heizmann CW, Peabody TD, Simon MA, He TC
  TITLE     Increased expression of S100A6 is associated with decreased 
            metastasis and inhibition of cell migration and anchorage 
            independent growth in human osteosarcoma.
  JOURNAL   Cancer Lett. 2005 Nov 8. 229(1):135-48.
  PUBMED    16157226
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Lett. 2005 Nov 8. 229(1):135-48."
COMMENT     Sequence Label: pHAHA
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    134..268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(158..177)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     212..231
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(411..433)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cagagctcgt ttagtgaccg tcagatctct agaagctggg taccgaaatg ggatacgatg
       61 ttccagatta cgcttcgtac gatgttccag attacgcaag cttgctagcg gccgctcgag
      121 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
      181 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      241 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      301 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      361 ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta
      541 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
      601 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
      661 ccacatgtga cacggggggg g
//
