LOCUS       sequence-189744-        6804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.3.1
FEATURES             Location/Qualifiers
     source          1..6804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      5..460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(92..111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     302..323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        472..851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        852..1055
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1006..1026
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1100..1119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1100..1118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1513..1524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3013..3030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3019..3243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3375..3963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3455..3474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4292..4339
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4364..4383
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4571..5365)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4571..5365)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4682..4701)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5292..5311
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5400..5757)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5414..5549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5566..5757
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5714..5734
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(5817..6608,6609..6677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6440..6459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6678..6782)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
       61 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
      241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
      301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      421 tttaacgcga attttaacaa aatattaacg cttacaattt acgcgcgcgt tgacattgat
      481 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      541 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      601 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      661 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      721 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      781 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      841 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      901 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      961 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     1021 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     1081 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtt
     1141 taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga attcggcttc
     1201 accatgggtt gctctttctc tatcttcctt ctggctttgc tctcttgcct gactgtgccc
     1261 gcgtcagcct accaagtacg caactcctcg ggcctttatc atgtcaccaa tgattgccct
     1321 aactcgagta ttgtgtacga gacgaccgac agcatcctgc attctccggg gtgtgtccct
     1381 tgcgttcgcg aaggtgacgc ctcgaaatgt tgggtgccag tggcccccac ggtcgccacc
     1441 agggacggca aactccccac aacgcagctt cgacgtcaca tcgatctgct tgtcgggggt
     1501 gccaccctct gctcggccct ctatgtgggg gacttgtgtg ggtctgtctt tcttgtcggt
     1561 caactgttca ctttctcccc caggcgccac tggacgacgc aggactgcaa ctgttctatg
     1621 taccccggcc atataacggg tcaccgcatg gcatgggata tgatgatgaa ctggtctcct
     1681 acaacagcgt tggtagtagc tcagctgctc agggtcccgc aagccatctt ggacatgatc
     1741 gctggtgccc attggggagt cctagcgggc gtagcgtatt tctccatggt ggggaactgg
     1801 gcgaaggtcc tgctagtgct attgctgttt gctggcgtcg atgcggcaac ctataccacc
     1861 ggggggagtg ccgctaggac cacatctgta ctcagcggcc ttttcactca gggcgccaag
     1921 caggatatcc agctaatcaa caccaacggc agttggcaca tcaatcgtac tgccttgaac
     1981 tgtaacgcga gcctcgacac tggctgggta gcagggctct tctattacca taaattcaat
     2041 tcttcaggct gccccgagag gatggccagc tgtagacccc ttgccgattt cgatcaaggc
     2101 tggggcccca tcagctacgc caacggaagc ggccccgaac accgccccta ctgctggcac
     2161 taccccccaa agccttgtgg tatcgtgccg gcacagagtg tatgtggccc agtgtattgc
     2221 ttcaccccta gccccgtggt ggtgggaacg accgacagag cgggcgcgcc tacctacaac
     2281 tggggtaaca atgacacgga cgttttcgtc cttaacaaca ccagaccacc gttgggcaat
     2341 tggttcggtt gcacctggat gaactcatct ggatttacca aagtgtgcgg agcgcctcct
     2401 tgtgtcatcg gaggggtggg caacaacacc ttgcactgcc ccactgactg tttccgcaag
     2461 catccggaag ccacatactc tcggtgtggc tccggaccct ggatcacgcc caggtgtcta
     2521 gtccactact cctacagact ttggcattat ccttgtacca tcaattacac cctgttcaaa
     2581 gtcaggatgt acgtgggagg ggttgagcac aggctggagg ttgcgtgcaa ctggacgcga
     2641 ggcgagcgtt gtgatctgga cgacagggac aggtccgagc tcagcccgct gctgctgtcc
     2701 accacacagt ggcaggtcct tccatgttcc ttcacgacct tgccagcttt gactaccggc
     2761 ctcatccacc tccatcagaa catcgtagac gtgcagtatt tgtacggggt ggggtcaagc
     2821 attgtgtcct gggccatcaa gtgggagtat gtcatcctct tgttcctcct gctcgcagac
     2881 gcgcgcatct gctcctgtct gtggatgatg ttactcatat cccaagcgga ggcgtaaaag
     2941 ccgaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
     3001 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3061 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     3121 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     3181 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3241 tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga atcaggggat
     3301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3361 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat ggcagggcct
     4141 gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg
     4201 gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag
     4261 tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc gtgcgtttta
     4321 ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt
     4381 tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     4441 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     4501 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     4561 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     4621 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     4681 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     4741 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     4801 catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt
     4861 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     4921 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     4981 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     5041 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     5101 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     5161 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     5221 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     5281 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     5341 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcgatct
     5401 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     5461 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     5521 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     5581 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     5641 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     5701 ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcaggac
     5761 tcttcctttt tcaataaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5821 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5881 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5941 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6001 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6061 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6121 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6181 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6241 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6301 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6361 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6421 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6481 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6541 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6601 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6661 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6721 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6781 cgcacatttc cccgaaaagt gcca
//
