LOCUS       sequence-189747-        5403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF473 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF473
FEATURES             Location/Qualifiers
     source          1..5403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2714..2813)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2830..2849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2831..2849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2854..2870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2854..2870)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3301..4092
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4185..4773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4674..4693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4927..4944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5103..5130)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5222..5308)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5357..5379
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5371..5388
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5372..5388
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggctgagga atttgtgacc
      121 ctcaaggatg tcggcatgga cttcaccttg ggagactggg agcagctcgg gctggaacag
      181 ggggacacgt tctgggacac agcgttggac aattgccagg acctcttcct gctggacccc
      241 ccaagaccca acctgacctc ccacccagat ggcagtgaag atctggagcc tctggcagga
      301 ggaagcccag aagcaacaag ccctgatgtg actgagacca agaactctcc tctgatggag
      361 gatttcttcg aagaaggatt ctcccaggag attatagaga tgttatccaa ggatggcttc
      421 tggaactcca atttcggaga agcctgtata gaggacacct ggttagatag tttgctaggc
      481 gatccagaaa gtcttctgag gtctgatatt gccaccaacg gggaaagtcc cacggaatgc
      541 aagagtcatg aattaaagag aggactcagt cctgtgtcca ccgtttccac gggagaagat
      601 tccatggtgc ataatgtttc tgaaaagacc ctcacaccag ctaagtctaa ggaatatagg
      661 ggtgagtttt tctcctactc cgaccacagc cagcaggatt ctgttcagga aggggagaaa
      721 ccatatcaat gtagtgaatg tgggaaaagc ttcagtggga gttaccgtct tacccagcac
      781 tggatcactc atactaggga gaaacccact gtccatcaag agtgtgagca aggttttgac
      841 cggaatgctt ccctttctgt gtatccgaaa actcacacgg gctacaaatt ctatgtgtgt
      901 aatgaatatg ggacaacttt tagtcagagt acatacctgt ggcatcagaa aactcacact
      961 ggagaaaaac catgtaagag tcaagatagt gaccacccac ccagtcatga cacacagcct
     1021 ggtgagcatc agaaaactca cacagatagt aagtcctaca actgtaacga atgcggcaag
     1081 gcttttaccc ggatcttcca ccttactcgg caccagaaga tccacactcg gaaacgctat
     1141 gagtgttcca agtgccaggc gaccttcaac ttgagaaaac acctcatcca acatcagaaa
     1201 actcacgctg caaaaactac ctctgagtgt caggagtgtg ggaagatttt taggcacagt
     1261 tcgctgctca ttgaacacca ggctcttcat gctggagagg agccttataa gtgtaacgaa
     1321 cgtgggaaat ccttcaggca taactctacc ctaaagatcc atcagagggt tcacagtgga
     1381 gagaagcctt acaaatgcag tgagtgtggg aaggccttcc accggcacac tcaccttaat
     1441 gaacatcggc gaattcatac aggctacaga ccccacaaat gtcaggaatg cgtcaggagt
     1501 ttcagccggc cctcacatct gatgcgacat caggccattc acaccgcaga aaagccctat
     1561 agctgtgctg aatgcaagga gactttcagc gataacaatc gccttgtgca acaccagaaa
     1621 atgcacactg tcaaaacccc atatgaatgt caggagtgcg gagaacgctt catttgcggc
     1681 tcaaccctga agtgccacga gagtgttcac gccagagaaa aacaaggatt ttttgtgagt
     1741 gggaagatct tggatcagaa cccagaacag aaagagaagt gctttaagtg taacaaatgt
     1801 gagaaaacct ttagctgcag caaataccta actcagcacg agaggattca caccagggga
     1861 gtgaagccct ttgaatgtga ccagtgtggg aaagcctttg gccaaagtac tcggctcatt
     1921 caccatcaaa gaatccactc tagagtgagg ctgtataaat ggggtgagca agggaaagcc
     1981 atcagcagtg cctcccttat caaacttcag tccttccaca caaaggagca cccttttaaa
     2041 tgtaacgaat gcggaaagac cttcagccac agtgcacacc tctcaaaaca tcagttaatt
     2101 cacgctggag agaatccctt taaatgtagt aagtgtgaca gagtcttcac ccagagaaac
     2161 taccttgttc agcatgagcg aactcatgcc agaaagaagc cgttggtgtg taacgaatgc
     2221 gggaaaacgt tccgtcagag ctcatgcctt tctaagcatc agagaattca ctcaggtgag
     2281 aagccctatg tatgtgatta ctgcgggaag gccttcggcc tgagtgctga gcttgtccgc
     2341 caccagagaa ttcacactgg agaaaagcct tatgtttgtc aggaatgcgg gaaagccttc
     2401 acccagagct catgcctttc tattcaccgg agagttcaca ctggggagaa gccctacaga
     2461 tgtggtgaat gtgggaaagc ctttgcccag aaagcaaatc taacacagca ccagagaatt
     2521 cacacagggg agaagcctta ctcctgtaat gtgtgtggca aagcttttgt cctcagtgcc
     2581 catctcaacc agcacctgag agttcacacc caggagacac tttatcagtg tcaacgttgc
     2641 cagaaagcct ttcggtgcca ctcgagcctc agccgccatc agcgtgtaca caacaagcag
     2701 caatactgcc tgtacccaac tttcttgtac aaagttggca ttataagaaa gcattgctta
     2761 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     2821 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2881 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     2941 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     3001 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     3061 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     3121 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     3181 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     3241 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
     3301 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     3361 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     3421 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     3481 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     3541 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     3601 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     3661 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     3721 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     3781 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     3841 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     3901 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     3961 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     4021 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     4081 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     4141 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4321 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4501 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4561 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4621 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4681 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4741 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4801 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4861 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4921 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4981 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5041 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     5101 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     5161 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     5221 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     5281 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     5341 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     5401 ccc
//
