LOCUS       sequence-189748-        6799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.4.1
FEATURES             Location/Qualifiers
     source          1..6799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(97..116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     307..328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        477..856
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        857..1060
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1011..1031
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1105..1124
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1105..1123
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3013..3030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3019..3243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3375..3963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3455..3474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4292..4339
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4364..4383
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4571..5365)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4571..5365)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4682..4701)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5292..5311
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5400..5757)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5414..5549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5566..5757
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5714..5734
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(5817..6608,6609..6677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6440..6459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6678..6782)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
       61 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      181 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      301 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      361 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      421 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttacgcg cgcgttgaca
      481 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      541 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      601 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      661 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      721 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      781 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      841 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      901 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      961 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1021 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1081 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     1141 gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattcc
     1201 accatgggtt gctctttctc tatcttcctc ctagccctgc tttcttgcct gactgtgccc
     1261 gcttcagctt accaagtgcg caactccacg gggctttacc atgtcaccaa tgattgccct
     1321 aactcgagta ttgtgtacga aacagctgat gccatcctgc acgctccggg gtgtgtccct
     1381 tgcgttcgcg agggcaacgt ctcgaggtgt tgggcggcga tgacccccac ggtggccact
     1441 agggatggca aactccccgc aacggagctt cgacgacaca tcgatctgct tgtcgggggt
     1501 gccaccctct gctcggccct ctacgtgggg gacttgtgcg ggtctgtctt tcttgtcggt
     1561 cagctgttta ccctctctcc cagacgccac tggacgacgc aagactgcaa ctgttctatc
     1621 tatcccggcc acataacggg tcatcgcatg gcatgggata tgatgatgaa ctggtcccct
     1681 acgacggcat tggtagtagc tcagctgctc cggatcccac aagccatctt ggatatgatc
     1741 gctggtgctc actggggagt cctagcgggc atagcgtatt tctccatggt gggaaactgg
     1801 gcgaaggtcc tggtagtgct gctgctattc gccggcgttg acgcggagac ccgcgtctcc
     1861 gggggagctg ccgctcgcca ggcggcggga ttcagcagtc tttttacatc gggcgccaag
     1921 cagaacatcc agctgatcaa ctccaatggc agttggcaca tcaataggac ggccctgaac
     1981 tgtaacgaca gccttaccac cggctggata gcagggcttc tctaccacca caaattcaac
     2041 tcttcaggct gccccgagag aatggccagc tgtcgacccc ttaccgattt tgaccagggc
     2101 tggggcccta tcggctacac caatggaggc ggccccgacc atcgccccta ctgctggcac
     2161 taccccccaa aaccttgtgg tattgtgccc gcattgagcg tctgtggccc ggtgtactgt
     2221 ttcactccaa gtcccgtagt ggtgggaacg accgacaagt cgggcgcgcc tacctacaga
     2281 tggggtgaga atgaaacgga cgttttcgtc ctgaacaaca ctaggccacc gcttggcaat
     2341 tggttcggct gcacctggat gaacgcgact ggatatacca aagtttgcgg agcgccccct
     2401 tgtaacatcg gaggggtggg caacaacacc ttgcattgcc ccaccgattg tttccgcaag
     2461 catccggacg ccacatactc tcggtgcggc tccggcccct ggatcacacc caggtgcctg
     2521 gttgactacc cttacagact ttggcattat ccttgtactg tcaactacac catatttaag
     2581 gtcaggatgt acgtgggagg ggtcgagcac aggctggacg ctgcctgcaa ctggacacgg
     2641 ggcgagcgct gcgatctgga agacagggac aggtccgagc tcagcccgct gctgctgtcc
     2701 accacacagt ggcaagtcct tccgtgttca ttcacgaccc tgccggcctt gtccaccggc
     2761 ctcatccatc tccaccagaa cactgtggac gtgcagtact tgtacggagt ggggtcaagc
     2821 attgcgtcct gggccgtcaa gtgggaatac gttgttctcc tgttcctttt gctcgcggac
     2881 gcgcgcgtct gctcctgctt gtggatgatg ttactcatat cccaagcgga ggcgtaaaag
     2941 ccgaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
     3001 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3061 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     3121 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     3181 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3241 tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga atcaggggat
     3301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3361 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat ggcagggcct
     4141 gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg
     4201 gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag
     4261 tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc gtgcgtttta
     4321 ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt
     4381 tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     4441 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     4501 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     4561 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     4621 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     4681 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     4741 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     4801 catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt
     4861 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     4921 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     4981 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     5041 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     5101 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     5161 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     5221 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     5281 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     5341 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcgatct
     5401 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     5461 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     5521 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     5581 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     5641 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     5701 ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcaggac
     5761 tcttcctttt tcaataaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5821 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5881 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5941 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6001 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6061 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6121 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6181 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6241 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6301 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6361 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6421 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6481 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6541 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6601 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6661 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6721 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6781 cgcacatttc cccgaaaag
//
