LOCUS       sequence-189752-        6804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.4.2
FEATURES             Location/Qualifiers
     source          1..6804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(103..122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     313..334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        483..862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        863..1066
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1017..1037
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1111..1130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1111..1129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3024..3041)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3030..3254
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3386..3974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3466..3485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4303..4350
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4375..4394
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4582..5376)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4582..5376)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4693..4712)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5303..5322
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5411..5768)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5425..5560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5577..5768
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5725..5745
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(5828..6619,6620..6688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6451..6470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6689..6793)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
       61 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      121 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      181 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      241 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      301 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      361 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      421 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tacgcgcgcg
      481 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      541 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      601 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      661 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      721 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      781 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      841 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      901 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      961 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1021 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     1081 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     1141 tggctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg
     1201 aattcggctt caccatgggt tgctctttct ctatcttcct cctagccctg ctttcttgcc
     1261 tgactgtgcc cgcttcagct taccaagtgc gcaactccac ggggctttac catgtcacca
     1321 atgattgccc taactcgagt attgtgtacg aaacagctga tgccatcctg cacgctccgg
     1381 ggtgtgtccc ttgcgttcgc gagggcaacg tctcgaggtg ttgggcggcg atgaccccca
     1441 cggtggccac tagggatggc aaactccccg caacggagct tcgacgacac atcgatctgc
     1501 ttgtcggggg tgccaccctc tgctcggccc tctacgtggg ggacttgtgc gggtctgtct
     1561 ttcttgtcgg tcagctgttt accctctctc ccagacgcca ctggacgacg caagactgca
     1621 actgttctat ctatcccggc cacataacgg gtcatcgcat ggcatgggat atgatgatga
     1681 actggtcccc tacgacggca ttggtagtag ctcagctgct ccggatccca caagccatct
     1741 tggatatgat cgctggtgct cactggggag tcctagcggg catagcgtat ttctccatgg
     1801 tgggaaactg ggcgaaggtc ctggtagtgc tgctgctatt cgccggcgtt gacgcggaga
     1861 cccgcgtctc cgggggagct gccgctcgcc aggcggcggg attcagcagt ctttttacat
     1921 cgggcgccaa gcagaacatc cagctgatca actccagtgg cagttggcac atcaatagga
     1981 cggccctgaa ctgtaacgac agccttacca ccggctggat agcagggctt ctctaccacc
     2041 acaaattcaa ctcttcaggc tgtcccgaga gaatggccag ctgtcgaccc cttaccgatt
     2101 ttgaccaggg ctggggccct atcggctaca ccaatggaag cggccccgac catcgcccct
     2161 actgctggca ctacccccca aaaccttgtg gtattgtgcc cgcattgagc gtctgtggcc
     2221 cggtgtactg tttcactcca agtcccgtag tggtgggaac gaccgacaag tcgggcgcgc
     2281 ctacctacag atggggtgag aatgaaacgg acgttttcgt cctgaacaac actaggccac
     2341 cgcttggcaa ttggttcggc tgcacctgga tgaacgcgac tggatatacc aaagtttgcg
     2401 gagcgccccc ttgtaacatc ggaggggtgg gcaacaacac cttgcattgc cccaccgatt
     2461 gtttccgcaa gcatccggac gccacatact ctcggtgcgg ctccggcccc tggatcacac
     2521 ccaggtgcct ggttgactac ccttacagac tttggcatta tccttgtact gtcaactaca
     2581 ccatatttaa ggtcaggatg tacgtgggag gggtcgagca caggctggac gctgcctgca
     2641 actggacacg gggcgagcgc tgcgatctgg aagacaggga caggtccgag ctcagcccgc
     2701 tgctgctgtc caccacacag tggcaagtcc ttccgtgttc attcacgacc ctgccggcct
     2761 tgtccaccgg cctcatccat ctccaccaga acactgtgga cgtgcagtac ttgtacggag
     2821 tggggtcaag cattgcgtcc tgggccgtca agtgggaata cgttgttctc ctgttccttt
     2881 tgctcgcgga cgcgcgcgtc tgctcctgct tgtggatgat gttactcata tcccaagcgg
     2941 aggcgtaaaa gccgaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     3001 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3061 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3121 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     3181 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     3241 ggtgggctct atggcttctg aggcggaaag aaccagtggc ggtaatacgg ttatccacag
     3301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta
     4141 tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg
     4201 ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg
     4261 tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac
     4321 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     4381 ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc
     4441 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     4501 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     4561 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     4621 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     4681 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     4741 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     4801 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct
     4861 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     4921 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     4981 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     5041 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     5101 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     5161 cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag
     5221 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     5281 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     5341 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     5401 ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
     5461 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     5521 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     5581 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     5641 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     5701 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc tggttctttc
     5761 cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag taaacttggt
     5821 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5881 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5941 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6001 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6061 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6121 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6181 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6241 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6301 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6361 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6421 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6481 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6541 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6601 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6661 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6781 taggggttcc gcgcacattt cccc
//
