LOCUS       sequence-189753-        6799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.4.3
FEATURES             Location/Qualifiers
     source          1..6799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        479..858
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        859..1062
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1013..1033
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1107..1126
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1107..1125
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3015..3032)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3021..3245
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3377..3965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3457..3476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4294..4341
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4366..4385
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4573..5367)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4573..5367)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4684..4703)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5294..5313
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5402..5759)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5416..5551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5568..5759
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5716..5736
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(5819..6610,6611..6679))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6442..6461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6680..6784)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg cgcgcgttga
      481 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      541 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      601 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      661 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      721 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      781 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      841 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      901 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      961 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1021 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     1081 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
     1141 tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
     1201 cggcttcacc atgggttgct ctttctctat cttcctccta gccctgcttt cttgcctgac
     1261 tgtgcccgct tcagcttacc aagtgcgcaa ctccacgggg ctttaccatg tcaccaatga
     1321 ttgccctaac tcgagtattg tgtacgaaac agctgatgcc atcctgcacg ctccggggtg
     1381 tgtcccttgc gttcgcgagg gcaacgtctc gaggtgttgg gcggcgatga cccccacggt
     1441 ggccactagg gatggcaaac tccccgcaac ggagcttcga cgacacatcg atctgcttgt
     1501 cgggggtgcc accctctgct cggccctcta cgtgggggac ttgtgcgggt ctgtctttct
     1561 tgtcggtcag ctgtttaccc tctctcccag acgccactgg acgacgcaag actgcaactg
     1621 ttctatctat cccggccaca taacgggtca tcgcatggca tgggatatga tgatgaactg
     1681 gtcccctacg acggcattgg tagtagctca gctgctccgg atcccacaag ccatcttgga
     1741 tatgatcgct ggtgctcact ggggagtcct agcgggcata gcgtatttct ccatggtggg
     1801 aaacggggcg aaggtcctgg tagtgctgct gctattcgcc ggcgttgacg cggagacccg
     1861 cgtctccggg ggagctgccg ctcgccaggc ggcgggattc agcagtcttt ttacatcggg
     1921 cgccaagcag aacatccagc tgatcaactc caatggcagt tggcacatca ataggacggc
     1981 cctgaactgt aacgacagcc ttaccaccgg ctggatagca gggcttctct accaccacaa
     2041 attcaactct tcaggctgtc ccgagagaat ggccagctgt cgacccctta ccgattttga
     2101 ccagggctgg ggccctatcg gctacaccaa tggaagcggc cccgaccatc gcccctactg
     2161 ctggcactac cccccaaaac cttgtggtat tgtgcccgca ttgagcgtct gtggcccggt
     2221 gtactgtttc actccaagtc ccgtagtggt gggaacgacc gacaagtcgg gcgcgcctac
     2281 ctacagatgg ggtgagaatg aaacggacgt tttcgtcctg aacaacacta ggccaccgct
     2341 tggcaattgg ttcggctgca cctggatgaa cgcgactgga tataccaaag tttgcggagc
     2401 gcccccttgt aacatcggag gggtgggcaa caacaccttg cattgcccca ccgattgttt
     2461 ccgcaagcat ccggacgcca catactctcg gtgcggctcc ggcccctgga tcacacccag
     2521 gtgcctggtt gactaccctt acagactttg gcattatcct tgtactgtca actacaccat
     2581 atttaaggtc aggatgtacg tgggaggggt cgagcacagg ctggacgctg cctgcaactg
     2641 gacacggggc gagcgctgcg atctggaaga cagggacagg tccgagctca gcccgctgct
     2701 gctgtccacc acacagtggc aagtccttcc gtgttcattc acgaccctgc cggccttgtc
     2761 caccggcctc atccatctcc accagaacac tgtggacgtg cagtacttgt acggagtggg
     2821 gtcaagcatt gcgtcctggg ccgtcaagtg ggaatacgtt gttctcctgt tccttttgct
     2881 cgcggacgcg cgcgtctgct cctgcttgtg gatgatgtta ctcatatccc aagcggaggc
     2941 gtaagaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta
     3001 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3061 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3121 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3181 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     3241 tatggcttct gaggcggaaa gaaccagtgg cggtaatacg gttatccaca gaatcagggg
     3301 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3541 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3781 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4021 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4081 aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc
     4141 ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg
     4201 gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag
     4261 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt
     4321 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
     4381 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga
     4441 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     4501 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
     4561 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     4621 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     4681 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     4741 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     4801 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
     4861 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     4921 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     4981 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     5041 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     5101 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     5161 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt
     5221 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     5281 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     5341 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat
     5401 ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     5461 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     5521 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     5581 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     5641 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     5701 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg
     5761 actcttcctt tttcaataaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5821 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5881 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5941 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6001 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6061 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6121 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6181 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6241 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6301 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6361 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6421 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6481 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6541 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6601 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6661 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6721 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6781 cgcgcacatt tccccgaaa
//
