LOCUS       sequence-189756-        8943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+]..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Du Z, Crow ET, Kang HS, Li L
  TITLE     Distinct subregions of Swi1 manifest striking differences in prion 
            transmission and SWI/SNF function.
  JOURNAL   Mol Cell Biol. 2010 Oct;30(19):4644-55. doi: 10.1128/MCB.00225-10. 
            Epub 2010 Aug 2.
  PUBMED    20679490
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00225-10"; journalName: "Mol Cell Biol"; date: 
            "2010-10"; volume: "30"; issue: "19"; pages: "4644-55"
COMMENT     Sequence Label: p415TEF-NQYFP
FEATURES             Location/Qualifiers
     source          1..8943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        172..570
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     regulatory      2212..2221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2218..2220,2221..2223,2224..2937)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2263..2284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2524..2543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2871..2892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2950..3197
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2966..2984)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3215..3234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3216..3234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3244..3261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3244..3260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3253..3275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3401..3856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3488..3507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3698..3719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4157..4564
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4565..5659
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(6153..6172)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6272..6294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6332..6350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6392..6895
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6932..7036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7037..7105,7106..7897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7255..7274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8068..8656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8557..8576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8810..8827
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcatagcttc
      181 aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta
      241 ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt
      301 cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg tttctttttc
      361 ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt
      421 tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcttcaat
      481 ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat
      541 tagaaagaaa gcatagcaat ctaatctaag ttttctagaa ctagtatgga tttctttaat
      601 ttgaataata ataataataa taataatact actactacta ctactactac caataacaat
      661 aatactaata ataataatac taataataat aataatcctg ctaataatac caataacaac
      721 aatagcacag gccatagtag taatactaat aacaatacta ataacaacaa tacgaatacc
      781 ggagccagtg gtgtagacga ttttcagaat tttttcgacc caaaaccttt tgaccagaat
      841 ttggattcca acaataacaa cagtaatagt aataacaatg ataacaataa cagtaacacg
      901 gtagcctcca gcacgaactt tacttctcca acagcagtcg ttaataatgc cgctcctgct
      961 aatgttaccg gtggaaaagc tgctaatttc attcagaacc agtctcccca attcaattct
     1021 ccatatgact caaacaattc caacacaaat ttaaacagcc tgtctcctca ggctatttta
     1081 gccaagaatt caatcattga ttcttccaat ttacctcttc aagctcaaca acagctatat
     1141 ggcggtaaca ataataataa tagcaccgga attgcgaacg ataatgtaat aacaccccat
     1201 tttatcacca atgttcaatc catcagtcaa aattcctcat cttctactcc gaacacaaac
     1261 tctaactcta ctccgaatgc aaatcaacaa tttttgccat tcaataacag tgcttccaat
     1321 aatggtaatt tgacgtctaa ccagcttatt tctaattacg ctgcttcaaa ttcaatggac
     1381 agatcatcct ctgcaagcaa tgaatttgtt ccgaacacaa gtgacaacaa caacaacagt
     1441 aataaccata atatgcgtaa taacagcaat aataaaacga gcaataacaa taatgtcact
     1501 gccgtgcctg ctgcaactcc cgctaatacc aataattcaa cttcaaacgc aaatacagtg
     1561 ttctctgaaa gagctgcaat gtttgccgct ttgcagcaaa agcagcagca acgttttcag
     1621 gctctacagc agcagcagca gcagcaacaa aatcaacaac agcaaaatca acaaccacaa
     1681 cagcaacaac agcaacaaca gaatcccaaa tttttgcaga gtcaacggca gcaacaacaa
     1741 agatctattt tacaaagtct gaacccggca ttacaagaga aaatatctac tgaattgaat
     1801 aacaaacaat atgaactttt tatgaaatct ttgattgaaa actgtaaaaa aagaaatatg
     1861 ccgttacaat caataccaga aatcggcaac agaaaaataa atctttttta tctttatatg
     1921 ttggttcaaa aattcggtgg tgcagatcaa gtaacaagga cccaacagtg gtctatggtg
     1981 gcccaaaggc tgcaaatttc cgattaccaa caattagaat caatctattt tagaatttta
     2041 ttaccttacg aaagacacat gatttcccaa gaaggtataa aggaaactca agcgaaaaga
     2101 atattcttgc aacagttctt acaagaacta ttgaagaaag ttcaacaaca gcagcaggcc
     2161 gctgcattgg ccaatgccaa caataacatt aatacccggg atccaccggt cgccaccatg
     2221 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2281 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2341 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2401 gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     2461 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2521 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2581 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2641 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2701 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2761 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2821 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2881 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     2941 ggcctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg
     3001 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
     3061 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
     3121 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
     3181 cgaaggcttt aatttgcggc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     3241 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3301 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3361 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     3421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3601 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3721 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3781 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3841 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3901 accgcatatc gaccggtcga ggagaacttc tagtatatct acatacctaa tattattgcc
     3961 ttattaaaaa tggaatccca acaattacat caaaatccac attctcttca aaatcaattg
     4021 tcctgtactt ccttgttcat gtgtgttcaa aaacgttata tttataggat aattatactc
     4081 tatttctcaa caagtaattg gttgtttggc cgagcggtct aaggcgcctg attcaagaaa
     4141 tatcttgacc gcagttaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat
     4201 ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca
     4261 cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat
     4321 acctttttca actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt
     4381 tcatatagaa tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac
     4441 aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct
     4501 aacttttctt accttttaca tttcagcaat atatatatat atatttcaag gatataccat
     4561 tctaatgtct gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat
     4621 cacagccgaa gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt
     4681 cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat gctacaggtg ttccacttcc
     4741 agatgaggcg ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg
     4801 tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga
     4861 acttcaattg tacgccaact taagaccatg taactttgca tccgactctc ttttagactt
     4921 atctccaatc aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt
     4981 gggaggtatt tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag
     5041 tgaacaatac accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct
     5101 acaacatgag ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc
     5161 aagattatgg agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt
     5221 tcaacatcaa ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa
     5281 tggtattata atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat
     5341 cccaggttcc ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac
     5401 cgcatttggt ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt
     5461 caaccctatc gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc
     5521 tgaagaaggt aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac
     5581 tggtgattta ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt
     5641 taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat
     5701 aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag
     5761 acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa
     5821 ggaatacagg taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac
     5881 tttaacatta atattgacaa ggaggagggc accacacaaa aagttaggtg taacagaaaa
     5941 tcatgaaact atgattccta atttatatat tggaggattt tctctaaaaa aaaaaaaata
     6001 caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct
     6061 tgaaccattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc
     6121 ttaacgacgt agtcgatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     6181 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6241 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6301 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     6361 aatgtcatga taataatggt ttcttaggac ggatcgcttg cctgtaactt acacgcgcct
     6421 cgtatctttt aatgatggaa taatttggga atttactctg tgtttattta tttttatgtt
     6481 ttgtatttgg attttagaaa gtaaataaag aaggtagaag agttacggaa tgaagaaaaa
     6541 aaaataaaca aaggtttaaa aaatttcaac aaaaagcgta ctttacatat atatttatta
     6601 gacaagaaaa gcagattaaa tagatataca ttcgattaac gataagtaaa atgtaaaatc
     6661 acaggatttt cgtgtgtggt cttctacaca gacaagatga aacaattcgg cattaatacc
     6721 tgagagcagg aagagcaaga taaaaggtag tatttgttgg cgatccccct agagtctttt
     6781 acatcttcgg aaaacaaaaa ctattttttc tttaatttct ttttttactt tctattttta
     6841 atttatatat ttatattaaa aaatttaaat tataattatt tttatagcac gtgatgaaaa
     6901 ggacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7021 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7081 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7141 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7201 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7261 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7321 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7381 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7441 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7501 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7561 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7621 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7681 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7741 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7801 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7861 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7921 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7981 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8701 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8761 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8821 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8881 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8941 aat
//
