LOCUS       sequence-189761-        4935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF544 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF544
FEATURES             Location/Qualifiers
     source          1..4935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2246..2345)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2362..2381)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2363..2381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2386..2402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2386..2402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2833..3624
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3717..4305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4206..4225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4459..4476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4635..4662)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4754..4840)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4889..4911
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4903..4920
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4904..4920
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggaagcacg ttctatgctg
      121 gttccacccc aggcatctgt gtgcttcgag gatgtggcta tggcattcac acaggaggag
      181 tgggaacagc tggacctggc ccagaggaca ctgtaccgag aggtgacact ggagacctgg
      241 gagcatattg tctccctggg gcttttcctt tccaaatctg atgtgatctc tcagctggag
      301 caagaagagg acctgtgcag ggcagagcag gaggcccccc gagactggaa agctaccctt
      361 gaggagaata ggttgaattc tgaaaaagat cgagctaggg aagaactatc ccaccacgtg
      421 gaagtgtaca ggagtggacc ggaggagcca ccctctttgg tattaggaaa agtgcaagat
      481 cagagcaacc agttaaggga acaccaggag aactccttga ggttcatggt actcacctca
      541 gagagactgt ttgctcaaag ggaacattgt gagcttgaac ttgggggagg ttattctcta
      601 ccttctactt taagccttct acctacaaca ttacctacaa gtacaggttt ccctaagccc
      661 aactcacaag ttaaagagtt gaaacaaaat tcagctttca ttaatcatga gaaaaatgga
      721 gcagatggga agcactgtga gagtcatcag tgtgctagag ctttctgtca gagtatttac
      781 ttgagtaaac ttggaaacgt tgaaacagga aagaaaaacc cttatgaata tattgtcagt
      841 ggtgactctc tcaactatgg ttcctccctt tgttttcatg gtagaacttt ttcagtgaag
      901 aaaagtgatg actgtaagga ttatggaaac ctcttcagtc acagtgtgtc tctgaatgaa
      961 cagaagccag tgcattttgg gaaaagtcag tatgagtgtg atgagtgcag ggaaacctgt
     1021 tctgagagtc tgtgccttgt acaaacagaa agaagtggcc ctggagagac ccccttcaga
     1081 tgtgaggaac gctgtgctgc cttccccatg gcctcatctt tttctgactg taacatcatt
     1141 cagactacag agaagccatc tgtgtgtaat cagtgtggaa aatctttcag ctgttgtaag
     1201 ctcatacacc agagaacaca cactggagaa aagcccttcg aatgtactca gtgtgggaaa
     1261 tcttttagcc agagctatga ccttgtcata catcagagga cacacactgg agagaagccc
     1321 tatgagtgtg acctgtgtgg gaaatccttc acccagagat ccaaacttat tacacatcag
     1381 cgaattcaca ctggagaaaa accgtatcag tgtattgaat gcagaaaatc cttcaggtgg
     1441 aactctaacc tcattgtaca tcagagaatt catactggag agaaaccgta tgagtgcact
     1501 cactgtggaa agtccttcag ccaaagctat gagttagtta cacataaaag aacgcacact
     1561 ggagaaaaac ccttcaaatg tactcagtgt gggaaatctt tcagccagaa gtatgacctt
     1621 gttgtacatc agaggacaca cactggagag aagccctatg agtgcaacct gtgtgggaaa
     1681 tccttctccc agagttccaa acttattacg catcagcgaa ttcacactgg agaaaaaccg
     1741 tatcagtgta ttgaatgtgg gaaatccttc agatggaact ctaacctcgt catacatcag
     1801 agaattcata ctggagagaa accgtacgat tgcactcact gtggaaagtc cttcagccaa
     1861 agctatcagt tagttgcaca taaaagaact cacactggag aaaagcccta tgaatgtaac
     1921 gagtgtggaa aagccttcaa tcgaagcact cagctcatca ggcatctgca aattcacact
     1981 ggggagaagc cgtacaaatg caatcagtgc aataaagcct ttgcaaggag ctcctacctt
     2041 gtgatgcatc agagaactca cactggtgag aaaccttttg agtgtagtca gtgtgggaaa
     2101 gccttttcag ggagctctaa ccttctttcc catcacagaa ttcattctgg agagaaaccc
     2161 tatgaatgta gtgactgtgg gaaatccttc cggcagcaat ctcaacttgt agtgcatcgg
     2221 cggacacata ctggagagaa accttaccca actttcttgt acaaagttgg cattataaga
     2281 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2341 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2401 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2461 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     2521 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     2581 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     2641 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     2701 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2761 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2821 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2881 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2941 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     3001 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     3061 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     3121 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     3181 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     3241 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     3301 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3361 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3421 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3481 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3541 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     3601 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     3661 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3721 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3781 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3841 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3901 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3961 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4021 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4081 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4141 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4201 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4261 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4321 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4381 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4441 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4501 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4561 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4621 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4681 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4741 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4801 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4861 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4921 cttaagctcg ggccc
//
