LOCUS       sequence-189763-        6816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.5.2
FEATURES             Location/Qualifiers
     source          1..6816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        468..847
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        848..1051
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1002..1022
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1096..1115
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1096..1114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3021..3038)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3027..3251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3383..3971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3463..3482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4300..4347
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4372..4391
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4579..5373)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4579..5373)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4690..4709)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5300..5319
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5408..5765)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5422..5557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5574..5765
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5722..5742
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(5825..6616,6617..6685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6448..6467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6686..6790)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttacgc gcgcgttgac attgattatt
      481 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      541 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      601 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      661 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      721 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      781 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      841 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      901 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      961 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1021 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     1081 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa
     1141 cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc ggcttcacca
     1201 tgggttgctc tttctctatc ttccttctgg ccctgctctc ctgtctgact gtgcccgctt
     1261 cagcctacca agtgcgcaac tctacggggc tctaccatgt caccaatgat tgccctaact
     1321 cgagcattgt gtacgaggcg gctgatacca tcctgcacac tccggggtgt gtcccttgcg
     1381 ttcgcgaggg taacgcctcg aggtgttggg tggcggtgac ccccacggtg gccaccaggg
     1441 acggcaggct ccccacaacg cagcttcgac gtcacatcga tctgcttgtc gggagcgcta
     1501 ccctctgttc ggccctctac gtgggggacc tgtgcgggtc tgtctttctt gtcggtcaac
     1561 tgttcacctt ctctcccagg cgccactgga cgacgcagga ctgcaactgt tccatctatc
     1621 ccggcgagat aacgggccac cgcatggcat gggatatgat gatgaactgg tcccctacga
     1681 cggcgctggt agtagctcag ctgctccgga tcccacaagc catcttggac atgatcgctg
     1741 gtgctcactg gggagtcctg gcgggcatag cgtatttctc catggtgggg aactggacga
     1801 aggtcctggc agtgctgctg ctatttgccg gcgtcgacgc ggagactgtc gtctccgggg
     1861 gaagtgccgc tcgcgccacg gctggactca ctagtctctt ctcaccaggc gcccagcaaa
     1921 acatccagct gatcaacacc aacggcagtt ggcatatcaa ccgcacggcc ttgaactgca
     1981 atgatagcct tcacaccggc tggatatcag ggcttttcta ttccaacaga ttcaactctt
     2041 caggctgttc cgagaggttg gccagctgcc gacccctcac cgattttgac cagggctggg
     2101 gccctatcag ttatgtcaat gggagcggcc ccgaccaacg cccctactgc tggcactacc
     2161 ccccaaaacc ttgtggtatt gtgcccgcaa agagcgtgtg tggcccagta tactgcttca
     2221 ctcccagccc tgtggtggtg ggaacgaccg acaggtcggg cgcgcccacc tatgactggg
     2281 gtgaaaatga gacggacgct ttcgtcctca acaacaccag gccaccgatg ggcaattggt
     2341 tcggttgtac ctggatgaac tcaactggat tcaccaaggt gtgcggagcg gccccttgtg
     2401 ccatcggagg ggtgggcaac aaaaccttgt actgccccac tgattgtttc cgcaaacatc
     2461 cggaagccac gtactctcgg tgcggctccg gtccttggct cacacccagg tgcctggtcc
     2521 actaccagta taggctttgg cattatcctt gtaccatcaa tttcaccata tttaaagtca
     2581 ggatgtacgt gggaggggtc gagcacaggc tggaagctgc ctgcaactgg acgcggggcg
     2641 agcgttgtga tctggaagat agggacaggt ccgagctcag cccgttgctg ctgtccacca
     2701 cacagtggca ggttcttccg tgttccttca cgaccctgcc agccttgtcc accggtctca
     2761 tccacctcca ccagaacatt gtggacgtgc agtacctgta cggggtgggg tcaagcatcg
     2821 tgtcctgggc catcaagtgg gagtacgtcg ttctcctgtt tcttctgctt gcagacgcgc
     2881 gcgtctgctc ctgcttgtgg atgatgttac tcatagccca agctgaggcc taatctagaa
     2941 actttaagcc gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     3001 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3061 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3121 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3181 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3241 gggctctatg gcttctgagg cggaaagaac cagtggcggt aatacggtta tccacagaat
     3301 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3361 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3421 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3481 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3541 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3601 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3661 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3721 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3781 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3841 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3901 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3961 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4021 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4081 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg
     4141 cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt
     4201 ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat
     4261 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt
     4321 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct
     4381 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc
     4441 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     4501 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     4561 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     4621 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     4681 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     4741 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     4801 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc
     4861 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     4921 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     4981 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     5041 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     5101 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     5161 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
     5221 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     5281 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     5341 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     5401 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     5461 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     5521 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     5581 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     5641 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     5701 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
     5761 ctcaggactc ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg
     5821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6781 gggttccgcg cacatttccc cgaaaagtgc cacctg
//
