LOCUS       sequence-189767-        5127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF41 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF41
FEATURES             Location/Qualifiers
     source          1..5127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2438..2537)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2554..2573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2555..2573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2578..2594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2578..2594)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3025..3816
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3909..4497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4398..4417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4651..4668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4827..4854)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4946..5032)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5081..5103
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5095..5112
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5096..5112
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggcagctaa tggggactct
      121 cccccatggt ccccggccct ggctgcagag ggacgtggca gctcatgtga ggcttcagtg
      181 tcatttgagg acgtgactgt ggacttcagc aaggaggagt ggcagcactt ggaccctgcc
      241 cagagacgcc tgtactggga tgtgacacta gagaactaca gccacctgct ctcagtgggg
      301 taccaaattc ccaagtcaga ggctgccttc aagttggagc aaggagaggg gccatggatg
      361 ctggaggggg aagccccaca tcagagctgt tcaggtgagg ctattgggaa aatgcagcaa
      421 cagggaattc ctggaggaat tttcttccac tgtgagagat ttgatcaacc cataggagaa
      481 gattcattat gttctatttt agaagaactg tggcaagata atgaccagct agagcaacgt
      541 caggaaaacc agaataacct tttaagtcat gtgaaagtat tgattaagga gaggggctat
      601 gaacataaaa acattgaaaa aataattcat gtgactacca agcttgttcc ttcaattaaa
      661 agactccata actgtgacac aattttgaag catactttaa actcacataa tcataataga
      721 aacagtgcaa caaagaacct tggcaagatt tttggaaatg gtaacaattt cccccatagc
      781 ccttcctcta ctaagaatga gaatgctaaa acaggagcaa attcctgtga acatgaccac
      841 tatgaaaaac atctcagcca caaacaagct cccacccacc atcagaaaat tcatcctgag
      901 gagaagcttt atgtgtgtac tgaatgtgta atgggcttca ctcagaagtc acatctgttt
      961 gagcatcaga gaattcatgc tggagaaaag tcccgtgaat gtgacaaaag caacaaagtc
     1021 ttcccccaga aaccccaggt tgatgtacat ccaagtgttt atacaggaga aaaaccctat
     1081 ctgtgtactc aatgtgggaa agtctttacc ctcaaatcaa acctcattac acatcaaaaa
     1141 attcataccg ggcagaaacc ctacaaatgc agtgaatgtg gaaaagcctt tttccagaga
     1201 tcagacctct ttagacatct gagaattcat acaggagaaa aaccttatga atgcagtgaa
     1261 tgtggaaaag gcttctccca gaactcagac ctcagtatac atcagaaaac tcataccgga
     1321 gagaaacact atgaatgcaa tgaatgtggg aaggctttca caagaaaatc agcactcagg
     1381 atgcatcaga gaatccacac gggagagaaa ccttatgtat gcgctgactg tgggaaggcc
     1441 ttcatccaga aatcacattt caacacacat cagagaattc atactggaga aaagccgtat
     1501 gaatgcagtg actgtgggaa atccttcact aagaagtcac aactccatgt gcatcaaaga
     1561 attcacaccg gagagaaacc ctatatatgt acagaatgtg gaaaggtctt cactcacagg
     1621 acaaacctca ccacacatca gaaaactcat actggggaaa aaccctatat gtgtgctgaa
     1681 tgtggaaagg cttttactga ccagtcaaat ctcattaaac accagaaaac tcacactgga
     1741 gagaaaccct ataagtgcaa tggctgtgga aaagccttca tatggaagtc gcgcctcaaa
     1801 atacatcaga aatctcatat tggagagaga cactatgaat gcaaggactg cgggaaagcc
     1861 ttcatccaga aatcaacact aagcgtgcat cagagaatcc atacaggaga gaaaccgtac
     1921 gtttgtcctg aatgcgggaa ggcctttatc cagaaatcgc acttcattgc gcatcataga
     1981 atccatactg gagagaagcc ttatgaatgc agcgactgtg ggaaatgctt cactaagaag
     2041 tcacaactcc gtgtgcatca gaaaatccac acaggtgaga agcccaatat atgtgctgaa
     2101 tgtggaaagg ccttcactga ccgatcaaat ctcataacac atcagaaaat ccacactagg
     2161 gagaaaccct atgaatgtgg tgactgcggg aaaaccttca cctggaagtc acgcctcaat
     2221 atacatcaga agtctcatac tggagaaaga cactatgaat gtagtaaatg tgggaaagct
     2281 ttcatccaga aagccacact aagtatgcat cagataattc atacaggaaa gaaaccttat
     2341 gcttgtacag aatgtcagaa ggcctttact gacagatcga atctcattaa acaccagaaa
     2401 atgcatagtg gagaaaaacg ctataaagcc agtgactgcc caactttctt gtacaaagtt
     2461 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2521 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2581 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2641 gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg
     2701 ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     2761 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     2821 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2881 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     2941 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     3001 cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa
     3061 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     3121 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     3181 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     3241 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     3301 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     3361 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     3421 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     3481 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     3541 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     3601 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     3661 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     3721 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     3781 gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa
     3841 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4681 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4741 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     4801 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     4861 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     4921 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     4981 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     5041 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     5101 acgacggcca gtcttaagct cgggccc
//
