LOCUS       sequence-189771-        6804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.5.3
FEATURES             Location/Qualifiers
     source          1..6804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(77..96)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     287..308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        457..836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        837..1040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     991..1011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1085..1104
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2998..3015)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3004..3228
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3360..3948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3440..3459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4277..4324
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4349..4368
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4556..5350)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4556..5350)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4667..4686)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5277..5296
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5385..5742)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5399..5534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5551..5742
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5699..5719
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(5802..6593,6594..6662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6425..6444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6663..6767)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(6794..6804,1..445)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
       61 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      121 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      181 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      241 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
      301 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
      361 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
      421 aacaaaatat taacgcttac aatttacgcg cgcgttgaca ttgattattg actagttatt
      481 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      541 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      601 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      661 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      721 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      781 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      841 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      901 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      961 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     1021 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
     1081 aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg
     1141 taccgagctc ggatccacta gtccagtgtg gtggaattcg gcttcaccat gggttgctct
     1201 ttctctatct tccttctggc cctgctctcc tgtctgactg tgcccgcttc agcctaccaa
     1261 gtgcgcaact ctacggggct ctaccatgtc accaatgatt gccctaactc gagcattgtg
     1321 tacgaggcgg ctgataccat cctgcacact ccggggtgtg tcccttgcgt tcgcgagggt
     1381 aacgcctcga ggtgttgggt ggcggtgacc cccacggtgg ccaccaggga cggcaggctc
     1441 cccacaacgc agcttcgacg tcacatcgat ctgcttgtcg ggagcgctac cctctgttcg
     1501 gccctctacg tgggggacct gtgcgggtct gtctttcttg tcggtcaact gttcaccttc
     1561 tctcccaggc gccactggac gacgcaggac tgcaactgtt ccatctatcc cggcgacata
     1621 acgggccacc gcatggcatg ggatatgatg atgaactggt cccctacgac ggcgctggta
     1681 gtagctcagc tgctccggat cccacaagcc atcttggaca tgatcgctgg tgctcactgg
     1741 ggagtcctgg cgggcatagc gtatttctcc atggtgggga actggacgaa ggtcctggca
     1801 gtgctactgc tatttgccgg cgtcgacgcg gagactgtcg tctccggggg aagtgccgct
     1861 cgcgccacgg ctggactcac tagtctcttc tcaccaggcg cccagcaaaa catccagctg
     1921 atcaacacca acggcagttg gcatatcaac cgcacggcct tgaactgcaa tgatagcctt
     1981 cataccggct ggatagcagg gcttttctat tccaacagat tcaactcttc aggctgttcc
     2041 gagaggttgg ccagctgccg acccctcacc gattttgacc agggctgggg ccctatcagt
     2101 tatgtcaatg ggagcggccc cgaccaacgc ccctactgct ggcactaccc cccaaaacct
     2161 tgtggtattg tgcccgcaaa gagcgtgtgt ggcccagtat actgcttcac tcccagccct
     2221 gtggtggtgg gaacgaccga caggtcgggc gcgcccacct acgactgggg tgaaaatgag
     2281 acggacgttt tcgtcctcaa caacaccagg ccaccgatgg gcaattggtt cggttgtacc
     2341 tggatgaact caactggatt caccaaggtg tgcggagcgg ccccttgtgc catcggaggg
     2401 gtgggcaaca aaaccttgta ctgccccact gattgtttcc gcaaacatcc ggaagccacg
     2461 tactctcggt gcggctccgg tccttggctc acacccaggt gcctggtcca ctaccagtat
     2521 aggctttggc attatccttg taccatcaat ttcaccgtat ttaaagtcag gatgtacgtg
     2581 ggaggggtcg agcacaggct ggaagctgcc tgcaactgga cgcggggcga gcgttgtgat
     2641 ctggaagata gggacaggtc cgagctcagc ccgttgctgc tgtccaccac acagtggcag
     2701 gttcttccgt gttccttcac gaccctgcca gccttgtcca ccggtctcat ccacctccac
     2761 cagaacattg tggacgtgca gtacctgtac ggggtggggt caagcatcgt gtcctgggcc
     2821 atcaagtggg agtacgtcgt tctcctgttt cttctgcttg cagacgcgcg cgtctgctcc
     2881 tgcttgtgga tgatgttact catatcccaa gcggaggcgt aaaagccgaa ttctgcagat
     2941 atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     3001 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3061 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3121 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3181 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3241 aaagaaccag tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4081 tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc cccgacgttg
     4141 gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg
     4201 cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac
     4261 cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg tctttttatt
     4321 gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc
     4381 ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg
     4441 tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct
     4501 cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga
     4561 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     4621 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     4681 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     4741 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     4801 gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     4861 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     4921 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     4981 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     5041 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     5101 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     5161 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     5221 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     5281 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     5341 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca aaagcctagg
     5401 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     5461 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     5521 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     5581 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     5641 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     5701 cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc ctttttcaat
     5761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6781 aaagtgccac ctgacgcgcc ctgt
//
