LOCUS       sequence-189779-        5928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Del Vecchio AM, Romanczuk H, Howley PM, Baker CC
  TITLE     Transient replication of human papillomavirus DNAs.
  JOURNAL   J Virol. 1992 Oct . 66(10):5949-58.
  PUBMED    1326651
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1992 Oct . 66(10):5949-58."
COMMENT     Sequence Label: p2089 HPV-6b E2
FEATURES             Location/Qualifiers
     source          1..5928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..64
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     56..73
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     57..73
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2012..2634
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2211..2230)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2658..2678)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2663..2992
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2843..2978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3031..3127
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(3203..3222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3204..3222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3236..3252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3236..3252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3249..3271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3260..3276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3284..3290,3291..3308,3309..3314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3329..3350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3467..3484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3638..4226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3718..3737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4397..5188,5189..5257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5020..5039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5258..5362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5389..5844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5476..5495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5686..5707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
       61 aacgacggcc agtgccaagc tgatctatac attgaatcaa tattggcaat tagccatatt
      121 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
      181 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagaattg
      841 tttttatttt taattttctt tcaaatactt ccatcgaatt cagatctatg gaagcaatag
      901 ccaagcgttt agatgcgtgc caggaacagt tgttagaact ttatgaagaa aacagtactg
      961 acctacacaa acatgtattg cattggaaat gcatgagaca tgaaagtgta ttattatata
     1021 aagcaaaaca aatgggccta agccacatag gaatgcaagt agtgccacca ttaaaggtgt
     1081 ccgaagcaaa aggacataat gccattgaaa tgcaaatgca tttagaatca ttattaagga
     1141 ctgagtatag tatggaaccg tggacattac aagaaacaag ttatgaaatg tggcaaacac
     1201 cacctaaacg ctgttttaaa aaacggggca aaactgtaga agttaaattt gatggctgtg
     1261 caaacaatac aatggattat gtggtatgga cagatgtgta tgtgcaggac aatgacacct
     1321 gggtaaaggt gcatagtatg gtagatgcta agggtatata ttacacatgt ggacaattta
     1381 aaacatatta tgtaaacttt gtaaaagagg cagaaaagta tgggagcacc aaacattggg
     1441 aagtatgtta tggcagcaca gttatatgtt ctcctgcatc tgtatctagc actacacaag
     1501 aagtatccat tcctgaatct actacataca cccccgcaca gacctccacc cttgtgtcct
     1561 caagcaccaa ggaagacgca gtgcaaacgc cgcctaggaa acgagcacga ggagtccaac
     1621 agtccccttg caacgccttg tgtgtggccc acattggacc cgtggacagt ggaaaccaca
     1681 acctcatcac taacaatcac gaccagcacc aaagacggaa caacagtaac agttcagcta
     1741 cgcctatagt gcaatttcaa ggtgaatcca attgtttaaa gtgttttaga tataggctaa
     1801 atgacagaca cagacattta tttgatttaa tatcatcaac gtggcactgg gcctcctcaa
     1861 aggcaccaca taaacatgcc attgtaactg taacatatga tagtgaggaa caaaggcaac
     1921 agtttttaga tgttgtaaaa atacccccta ccattagcca caaactggga tttatgtcac
     1981 tgcacctatt gtaatttgtc tagaggatcc cgggtggcat ccctgtgacc cctccccagt
     2041 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     2101 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     2161 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     2221 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     2281 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     2341 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     2401 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     2461 gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga
     2521 cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt
     2581 gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac
     2641 gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     2701 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     2761 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     2821 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     2881 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     2941 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcct
     3001 cgaggaactg aaaaaccaga aagttaactg gtaagtttag tctttttgtc ttttatttca
     3061 ggtcccggat ccggtggtgg tgcaaatcaa agaactgctc ctcagtggat gttgccttta
     3121 cttctaggcc tgtacggaag tgttacttct gctctaaaag ctgctgcagg tcgactctag
     3181 ggccgcgtac cgagctcgaa ttccctatag tgagtcgtat taaattcgta atcatgtcat
     3241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     5401 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5461 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5521 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5581 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5641 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5701 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5761 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5821 acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg
     5881 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaaggggg
//
