LOCUS       sequence-18978-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     Unpublished He lab plasmids
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAd-RFP
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    139..157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    181..199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(201..404)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(230..250)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        405..708
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    778..900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(789..808)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     843..862
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cttctcgagg cggccgcgtc gacggtaccg gatcccctga attcggtgtc ttctatggag
       61 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac agctcgatct
      121 ctatcactga tagggagatc tctatcactg atagggagat ctctatcact gatagggaga
      181 tctctatcac tgatagggag agctctgctt atatagacct cccaccgtac acgcctaccg
      241 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
      301 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
      361 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
      421 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
      481 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
      541 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
      601 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
      661 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
      721 tatgggctat gaactaatga ccccgtaatt gattactatt aataactaga gacgcgttaa
      781 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      841 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaaacaagtt
      901 aacaacaaca attgcattca ttttatgttt ca
//
