LOCUS       sequence-189787-        6291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of GluSnFR, a glutamate sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6291)
  TITLE     Baljit Khakh lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV.GfaABC1D.GluSnFr.SV40
FEATURES             Location/Qualifiers
     source          1..6291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1158..1176
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     1211..1270
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     2239..2260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2366..2387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2627..2646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2852..2881
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2885..3031
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    3058..3179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3069..3088)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3123..3142
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3341..3470
                     /label=AAV2 ITR
     primer_bind     complement(3489..3506)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3489..3505)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3498..3520)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3647..4102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3734..3753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3944..3965
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4128..4232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4233..4301,4302..5093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5264..5852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5753..5772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6140..6161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6176..6181,6182..6199,6200..6206)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6214..6230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6219..6241
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6238..6254
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6238..6254
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatct aacatatcct ggtgtggagt aggggacgct gctctgacag aggctcgggg
      241 gcctgagctg gctctgtgag ctggggagga ggcagacagc caggccttgt ctgcaagcag
      301 acctggcagc attgggctgg ccgcccccca gggcctcctc ttcatgccca gtgaatgact
      361 caccttggca cagacacaat gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca
      421 gcccccctca aatgccttcc gagaagccca ttgagcaggg ggcttgcatt gcaccccagc
      481 ctgacagcct ggcatcttgg gataaaagca gcacagcccc ctaggggctg cccttgctgt
      541 gtggcgccac cggcggtgga gaacaaggct ctattcagcc tgtgcccagg aaaggggatc
      601 aggggatgcc caggcatgga cagtgggtgg caggggggga gaggagggct gtctgcttcc
      661 cagaagtcca aggacacaaa tgggtgaggg gagagctctc cccatagctg ggctgcggcc
      721 caaccccacc ccctcaggct atgccagggg gtgttgccag gggcacccgg gcatcgccag
      781 tctagcccac tccttcataa agccctcgca tcccaggagc gagcagagcc agagcaggtt
      841 ggagaggaga cgcatcacct ccgctgctcg caagctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtacttaa tacgactcac tataggctag cctcgagaat tcggcttggg
     1201 gatatccacc atggagacag acacactcct gctatgggta ctgctgctct gggttccagg
     1261 ttccactggt gacagatctg ccgcaggcag cacgctggac aaaatcgcca aaaacggtgt
     1321 gattgtcgtc ggtcaccgtg aatcttcagt gcctttctct tattacgaca atcagcaaaa
     1381 agtggtgggt tactcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact
     1441 caacaaaccg gacttgcagg taaaactgat tccgattacc tcacaaaacc gtattccact
     1501 gctgcaaaac ggcactttcg attttgaatg tggttctacc accaacaacg tcgaacgcca
     1561 aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa
     1621 gggtggcgat atcaaagatt ttgccaacct gaaagacaaa gccgtagtcg tcacttccgg
     1681 cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat
     1741 catcagcgcc aaagatcacg gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc
     1801 ctttatgatg gatgacgtgc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa
     1861 ctgggaaatc gtcggcaagc cgcagtctca ggaggcctac ggttgtatgt tgcgtaaaga
     1921 tgatccgcag ttcaaaaagc tgatggatga caccatcgct caggtgcaga cctccggtga
     1981 agcggaaaaa tggtttgata agtggttcaa aaatccaatt ctggtgagcc acaacgtcta
     2041 tatcatggcc gacaagcaga ggaacggcat caaggcgaac ttcaagatcc gccacaacat
     2101 cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg
     2161 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaaacttt cgaaagaccc
     2221 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2281 cggcatggac gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga
     2341 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2401 gttcagcgtg tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt
     2461 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2521 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2581 cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2641 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2701 gggcatcgac ttcaaggagg acggcaacat cctggggcat aagctggagt acaactttaa
     2761 caaccccctg aacatgaatt tcgaactgtc agacgaaatg aaagcactgt tcaaagaacc
     2821 gaatgacaag gcactgaaac tgcaggtcga cgaacaaaaa ctcatctcag aagaggatct
     2881 gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa
     2941 ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct cccttatcat
     3001 cctcatcatg ctttggcaga agaagccacg ttaggcggcc gcttcgagca gacatgataa
     3061 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3121 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3181 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     3241 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc
     3301 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
     3361 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     3421 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa
     3481 cctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3541 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3601 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3721 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3781 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3841 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3901 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3961 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4021 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4081 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4201 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4261 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4321 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4381 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4441 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4501 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4561 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4621 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4681 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4741 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4801 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4861 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4921 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4981 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5041 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5881 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5941 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6001 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6061 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6121 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6181 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6241 gaaacagcta tgaccatgat tacgccagat ttaattaagg ccttaattag g
//
