LOCUS       sequence-189791-        1214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of PRKACA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Honeyman JN, Simon EP, Robine N, Chiaroni-Clarke R, Darcy DG, Lim 
            II, Gleason CE, Murphy JM, Rosenberg BR, Teegan L, Takacs CN, Botero
            S, Belote R, Germer S, Emde AK, Vacic V, Bhanot U, LaQuaglia MP, 
            Simon SM
  TITLE     Detection of a recurrent DNAJB1-PRKACA chimeric transcript in 
            fibrolamellar hepatocellular carcinoma.
  JOURNAL   Science. 2014 Feb 28;343(6174):1010-4. doi: 10.1126/science.1249484.
  PUBMED    24578576
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-02-28- 28"; volume: "343"; 
            issue: "6174"; pages: "1010-4"
COMMENT     Sequence Label: pcrDNA3.1-PRKACA
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      125..134
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 cgccgccacc atgggcaacg ccgccgccgc caagaagggc agcgagcagg agagcgtgaa
      181 agaattctta gccaaagcca aagaagattt tcttaaaaaa tgggaaagtc ccgctcagaa
      241 cacagcccac ttggatcagt ttgaacgaat caagaccctc ggcacgggct ccttcgggcg
      301 ggtgatgctg gtgaaacaca aggagaccgg gaaccactat gccatgaaga tcctcgacaa
      361 acagaaggtg gtgaaactga aacagatcga acacaccctg aatgaaaagc gcatcctgca
      421 agctgtcaac tttccgttcc tcgtcaaact cgagttctcc ttcaaggaca actcaaactt
      481 atacatggtc atggagtacg tgcccggcgg ggagatgttc tcacacctac ggcggatcgg
      541 aaggttcagt gagccccatg cccgtttcta cgcggcccag atcgtcctga cctttgagta
      601 tctgcactcg ctggatctca tctacaggga cctgaagccg gagaatctgc tcattgacca
      661 gcagggctac attcaggtga cagacttcgg tttcgccaag cgcgtgaagg gccgcacttg
      721 gaccttgtgc ggcacccctg agtacctggc ccctgagatt atcctgagca aaggctacaa
      781 caaggccgtg gactggtggg ccctgggggt tcttatctat gaaatggccg ctggctaccc
      841 gcccttcttc gcagaccagc ccatccagat ctatgagaag atcgtctctg ggaaggtgcg
      901 cttcccttcc cacttcagct ctgacttgaa ggacctgctg cggaacctcc tgcaggtaga
      961 tctcaccaag cgctttggga acctcaagaa tggggtcaac gatatcaaga accacaagtg
     1021 gtttgccaca actgactgga ttgccatcta ccagaggaag gtggaagctc ccttcatacc
     1081 aaagtttaaa ggccctgggg atacgagtaa ctttgacgac tatgaggaag aagaaatccg
     1141 ggtctccatc aatgagaagt gtggcaagga gttttctgag ttttagtaat gattaagcgg
     1201 ccgctcgagt ctag
//
