LOCUS       sequence-189795-        1382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) 
            chimeric protein from fibrolamellar hepatocellular carcinoma.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Honeyman JN, Simon EP, Robine N, Chiaroni-Clarke R, Darcy DG, Lim 
            II, Gleason CE, Murphy JM, Rosenberg BR, Teegan L, Takacs CN, Botero
            S, Belote R, Germer S, Emde AK, Vacic V, Bhanot U, LaQuaglia MP, 
            Simon SM
  TITLE     Detection of a recurrent DNAJB1-PRKACA chimeric transcript in 
            fibrolamellar hepatocellular carcinoma.
  JOURNAL   Science. 2014 Feb 28;343(6174):1010-4. doi: 10.1126/science.1249484.
  PUBMED    24578576
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-02-28- 28"; volume: "343"; 
            issue: "6174"; pages: "1010-4"
COMMENT     Sequence Label: pcrDNA3.1-Chimera
FEATURES             Location/Qualifiers
     source          1..1382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccgccgcc accatgggta aagactacta ccagacgttg ggcctggccc gcggcgcgtc
      181 ggacgaggag atcaagcggg cctaccgccg ccaggcgctg cgctaccacc cggacaagaa
      241 caaggagccc ggcgccgagg agaagttcaa ggagatcgct gaggcctacg acgtgctcag
      301 cgacccgcgc aagcgcgaga tcttcgaccg ctacggggag gaagtgaaag aattcttagc
      361 caaagccaaa gaagattttc ttaaaaaatg ggaaagtccc gctcagaaca cagcccactt
      421 ggatcagttt gaacgaatca agaccctcgg cacgggctcc ttcgggcggg tgatgctggt
      481 gaaacacaag gagaccggga accactatgc catgaagatc ctcgacaaac agaaggtggt
      541 gaaactgaaa cagatcgaac acaccctgaa tgaaaagcgc atcctgcaag ctgtcaactt
      601 tccgttcctc gtcaaactcg agttctcctt caaggacaac tcaaacttat acatggtcat
      661 ggagtacgtg cccggcgggg agatgttctc acacctacgg cggatcggaa ggttcagtga
      721 gccccatgcc cgtttctacg cggcccagat cgtcctgacc tttgagtatc tgcactcgct
      781 ggatctcatc tacagggacc tgaagccgga gaatctgctc attgaccagc agggctacat
      841 tcaggtgaca gacttcggtt tcgccaagcg cgtgaagggc cgcacttgga ccttgtgcgg
      901 cacccctgag tacctggccc ctgagattat cctgagcaaa ggctacaaca aggccgtgga
      961 ctggtgggcc ctgggggttc ttatctatga aatggccgct ggctacccgc ccttcttcgc
     1021 agaccagccc atccagatct atgagaagat cgtctctggg aaggtgcgct tcccttccca
     1081 cttcagctct gacttgaagg acctgctgcg gaacctcctg caggtagatc tcaccaagcg
     1141 ctttgggaac ctcaagaatg gggtcaacga tatcaagaac cacaagtggt ttgccacaac
     1201 tgactggatt gccatctacc agaggaaggt ggaagctccc ttcataccaa agtttaaagg
     1261 ccctggggat acgagtaact ttgacgacta tgaggaagaa gaaatccggg tctccatcaa
     1321 tgagaagtgt ggcaaggagt tttctgagtt ttagtaatga ttaagcggcc gctcgagtct
     1381 ag
//
