LOCUS       sequence-189808-         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knock eGFP into human VE-cad locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Bao X, Bhute VJ, Han T, Qian T, Lian X, Palecek SP
  TITLE     Human pluripotent stem cell-derived epicardial progenitors can 
            differentiate to endocardial-like endothelial cells.
  JOURNAL   Bioeng Transl Med. 2017 Jun;2(2):191-201. doi: 10.1002/btm2.10062. 
            Epub 2017 May 22.
  PUBMED    29170757
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/btm2.10062"; journalName: "Bioeng Transl Med"; date: 
            "2017-06"; volume: "2"; issue: "2"; pages: "191-201"
COMMENT     Sequence Label: VE-cad-eGFP Donor plasmid
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             275..286
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccctgcttg tttcaacagg gagaagttag tggcgctagc ccagtcgtta aggaagtcgt
       61 aatccacgtc agagtcggat gagtcggtgc ccagggagct gagggactcg gctatggact
      121 cggagccctc gtagccgtag atgtgcagcg tgtcgtaggg ggggccgtcg ccgtcgtggt
      181 ccgcctcgtc cttcttcacc tcgatcatgg ctgccatctc cccgggccct ccgtgtgccc
      241 caggcgcgtg cctcggtggc ttctgcacct gcgcatagag ggaaggccgg gcgtccagcg
      301 cgggccgcgg gggcttggcc ccgccgcggc gcaccgagtt gagcaccgac acatcgtagc
      361 tggtggtgtc catctcgccg ccgccctcct cgtcgtaggt gaccagctgc tcgtggatct
      421 ccggcacgct cttgccgtgc gcgcgggcct gcttccggag ccgccgccgc aggaagatga
      481 gcagggtgat cactgcagcc agggagccga gcagcagagt cagacaaggc tgggggcctg
      541 ggcggccccc catgccccca tccccact
//
