LOCUS       sequence-189815-        7250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.14.2
FEATURES             Location/Qualifiers
     source          1..7250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..69)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        241..620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     775..795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     869..888
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        869..887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2754..2795
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2805..2822
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2845..2862)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2851..3075
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3121..3549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3208..3227)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3418..3439
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3558..3578)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3563..3892
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3743..3878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3805..3824
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3959..4753
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4013..4032)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4623..4642
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4929..5050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4966..4985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5020..5039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5099..5115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5099..5115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5112..5134)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5123..5139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5147..5153,5154..5171,5172..5177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5192..5213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5330..5347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5501..6089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5581..5600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6260..7051,7052..7120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6883..6902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7121..7225)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
       61 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
      121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
      181 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      841 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      901 gctagttaag cttggtaccg agctcggatc cagtaccctt caccatgggt tgctctttct
      961 ctatcttcct tctggctctg ctctcttgct tgactgtgcc cgcctcagcc taccaagtgc
     1021 gcaactccac ggggctttat catgtcacca acgattgccc taactcgagt attgtgtacg
     1081 aggcggatga tgccatcctc cacactccgg ggtgtgtccc ttgcgtccgc gagggtaacg
     1141 cctcgaggtg ctgggtggcg atgaccccca cggtggccac caaggacggt aaactcccca
     1201 caacgcagct tcgaaggcac atcgatctgc ttgtcgggag tgccaccctc tgctcggccc
     1261 tctacgtggg agacctgtgc gggtctgtct ttcttgtcgg tcaactgttt accttctctc
     1321 ccaggcgcca ctggacgacg caggactgca attgttctat ctatcccggc cacataacgg
     1381 gtcaccgcat ggcatgggat atgatgatga actggtcccc cacagcggcg ttagtagtag
     1441 ctcagctgct ccggatccca caagccatct tggacatgat cgctggtgcc cactggggag
     1501 tcctggcggg catagcgtat ttctccatgg tggggaactg ggcgaaggtc ctggcggtgt
     1561 tgctgctatt tgccggcgtg gacgcgcaaa ctcgcatctc cgggggagcc gccgcccatt
     1621 ccgtgcaggg ttttaccagt ttcttcagcg taggctctcg ccagaacatc cagctggtca
     1681 acaccaacgg cagttggcac atcaaccgca cggccttgaa ctgcaatgag agcattgaca
     1741 ccggctggat agcagggctt ttctactaca acaaattcaa ctcttcaggc tgtcccgaga
     1801 ggttggccag ttgccgcccc cttacccatt ttgaccaggg ctggggccct atcaagtata
     1861 ccagtggaga agactccgac caacgcccct actgctggca ctacgcccca aaaccttgtg
     1921 gtattgtgcc cgcaaagagc gtgtgtggcc cggtatattg ttttacacct agccccgttg
     1981 tggtggggac gactgacagg tcgggcgtgc caacctacaa ctggggtgaa aatgaaacgg
     2041 acgtcttcgt ccttaacaac accaggccac cactgggcaa ttggttcggt tgcacctgga
     2101 tgaattcatc tgggttcacc aaagtgtgcg gggcccctcc ctgtatcatc aaagggtcgg
     2161 gcaacaaaac cttgcactgc cctactgatt gcttccgcaa gcatccggaa gccacgtact
     2221 ctcggtgcgg ctccggtccc tggattacac ccagatgcct ggtcagctat ccatacagac
     2281 tttggcatta tccatgcacc atcaactaca ccatatttaa agtcaggatg tacgtgggag
     2341 gggtcgagca caggctggag gctgcctgca actggacgcg gggcgagcgt tgtgatctag
     2401 aagacaggga caggtcagag ctcagcccgc tgctgctatc caccacacag tggcaggtcc
     2461 ttccgtgttc cttcacgacc ctgccagcct tgtccaccgg cctcatccac ctccaccaga
     2521 acattgtgga cgtgcagtac ttgtacgggg tggggtcaag catcgcgtct tgggccatta
     2581 agtgggagta cgtagttctc ctgttcctcc tgcttgcaga cgcgcgcgtc tgctcctgct
     2641 tgtggatgat gttactcata tcccaagcgg aggcgtaata aaagggtcaa gacaattctg
     2701 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc
     2761 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc
     2821 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     2881 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     2941 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3001 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3061 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     3121 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3181 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3241 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3301 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3361 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3421 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3481 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3541 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3601 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3661 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3721 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     3841 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     3901 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     3961 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4021 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4081 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4141 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4201 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4261 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4321 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4381 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4441 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     4501 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4561 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4621 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4681 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4741 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     4801 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4861 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4921 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4981 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5041 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     5101 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5161 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5221 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5281 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5341 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5401 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5461 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5521 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5581 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5641 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5701 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5761 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5821 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5881 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5941 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6001 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6061 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6121 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6181 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6241 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6301 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6361 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6421 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6481 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6541 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6601 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6661 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6721 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6781 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6841 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6901 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6961 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7021 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7081 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7141 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7201 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
//
