LOCUS       sequence-189833-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human E2F1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pET21 E2F1
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..77
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatggct agcatgactg
       61 gtggacagca aatgggtcgg atccgaattc accatggcct tggccggggc ccctgcgggc
      121 ggcccatgcg cgccggcgct ggaggccctg ctcggggccg gcgcgctgcg gctgctcgac
      181 tcctcgcaga tcgtcatcat ctccgccgcg caggacgcca gcgccccgcc ggctcccacc
      241 ggccccgcgg cgcccgccgc cggcccctgc gaccctgacc tgctgctctt cgccacaccg
      301 caggcgcccc ggcccacacc cagtgcgccg cggcccgcgc tcggccgccc gccggtgaag
      361 cggaggctgg acctggaaac tgaccatcag tacctggccg agagcagtgg gccagctcgg
      421 ggcagaggcc gccatccagg aaaaggtgtg aaatccccgg gggagaagtc acgctatgag
      481 acctcactga atctgaccac caagcgcttc ctggagctgc tgagccactc ggctgacggt
      541 gtcgtcgacc tgaactgggc tgccgaggtg ctgaaggtgc agaagcggcg catctatgac
      601 atcaccaacg tccttgaggg catccagctc attgccaaga agtccaagaa ccacatccag
      661 tggctgggca gc
//
