LOCUS       sequence-189852-        5114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-localized photoconvertible green-to-red 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Nowotschin S, Hadjantonakis AK
  TITLE     Use of KikGR a photoconvertible green-to-red fluorescent protein for
            cell labeling and lineage analysis in ES cells and mouse embryos.
  JOURNAL   BMC Dev Biol. 2009 Sep 9;9:49.
  PUBMED    19740427
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2009-09-9"; volume: "9"; pages: "49"
COMMENT     Sequence Label: pCX-H2B-KikGR
FEATURES             Location/Qualifiers
     source          1..5114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     331..353
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     415..434
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             476..847
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="EPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLK
                     QVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGE
                     LAKHAVSEGTKAVTKYTSAK"
     CDS             857..1543
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
     primer_bind     complement(1636..1655)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1701..1756
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1755..1774)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2117..2133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2117..2133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2130..2152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2141..2157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2165..2171,2172..2189,2190..2195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2210..2231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2290..2485
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2336..2471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2491..2625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2528..2547)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2582..2601
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2693..2710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2864..3452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2944..2963)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3623..4414,4415..4483))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4246..4265
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4484..4588)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4619..4998
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
       61 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      121 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
      181 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
      241 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
      301 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
      361 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
      421 tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgagcgg atgcagagcc
      481 agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa
      541 gaaaggcggc aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa
      601 ggttctgaag caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa
      661 ttcgtttgtg aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta
      721 caacaagcgc tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc
      781 tggggagttg gccaagcacg ccgtgtccga gggtactaag gccgtcacca agtacaccag
      841 cgctaaggat ccgcgaatgg tgagtgtgat tacatcagaa atgaagatcg agctgcgtat
      901 ggaaggcgct gtaaacgggc acaagttcgt gattacaggg aaaggaagtg gccagccttt
      961 cgagggaata cagaatgtgg acctgacagt catagagggc ggacctcttc cttttgcttt
     1021 cgatatcctg acaacagcat tccattacgg caaccgggta tttgtcgaat acccagaaga
     1081 aatagtagac tacttcaagc agtcgtttcc tgagggttat tcttgggaac gaagcatgag
     1141 ttacgaagac gggggaattt gcctcgccac aaacaatata acgatgaaga aagacggcag
     1201 caactgtttt gtcaatgaaa ttcgatttga tggtgtgaac tttcctgcca atggtccagt
     1261 tatgcagagg aagaccgtca aatgggagcc atccactgag aaaatgtatg tgcgtgatgg
     1321 agtgctgaag ggtgatgtaa acatggctct gttgcttcaa ggaggtggcc attaccgatg
     1381 tgacttcaga actacttaca aagcaaagaa ggttgtccag ttgccagact atcacttcgt
     1441 ggatcatcaa atggagataa caagccatga caaggattac aacaaggtta agctgtatga
     1501 gcacgctaaa gctcattccg ggctgccaag gctggccaag taaccgctcg aggaattcac
     1561 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     1621 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     1681 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     1741 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     1801 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     1861 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     1921 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     1981 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     2041 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     2101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2281 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     2461 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     2521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     2641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3301 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3481 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     4621 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4681 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4741 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4801 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4861 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4921 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     4981 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     5041 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     5101 ggggcggggg gggg
//
