LOCUS       sequence-189870-        9894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human JMJD2A with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Cloos PA, Christensen J, Agger K, Maiolica A, Rappsilber J, Antal T,
            Hansen KH, Helin K
  TITLE     The putative oncogene GASC1 demethylates tri- and dimethylated 
            lysine 9 on histone H3.
  JOURNAL   Nature. 2006 Jul 20. 442(7100):307-11.
  PUBMED    16732293
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jul 20. 442(7100):307-11."
COMMENT     Sequence Label: pCMV-HA-JMJD2A
FEATURES             Location/Qualifiers
     source          1..9894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        157..536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        537..740
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     691..711
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     737..761
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          852..1424
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1504..1530
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1543..1567
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4849..4873)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4972..4991)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5037..5092
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5091..5110)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5450..5466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5450..5466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5463..5485)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5474..5490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5498..5504,5505..5522,5523..5528))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5543..5564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5681..5698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5852..6440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5932..5951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6611..7402,7403..7471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7234..7253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7472..7576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7644..7662
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7700..7722)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7822..7841
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8035..8057
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8049..8066
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8050..8066
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8477..8622
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8679..9473
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8733..8752)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9343..9362
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9667..9686)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9711..9758
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 caacgtcggg gcggcaagcc ctgccatagc cacgggcccc gtgggttagg gacggcggat
       61 cgcggccctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
      121 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      181 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      241 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      301 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      361 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      421 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      481 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      541 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      601 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      661 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      721 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      781 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      841 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      901 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      961 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
     1021 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1081 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1141 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1201 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1261 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1321 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1381 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1441 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatca attcaccatg
     1501 gcatacccat acgatgttcc agattacgct agcggatcat caacaagttt gtacaaaaaa
     1561 gcaggctccg aattcgccct taaaatggct tctgagtctg aaactctgaa tcccagtgct
     1621 aggataatga ccttttatcc aactatggaa gagttccgaa acttcagtag atacattgcc
     1681 tacattgaat cccaaggagc tcatcgggca gggctagcca aggttgttcc tccaaaagag
     1741 tggaagccac gagcatccta tgatgacatt gatgatttgg tcattcctgc ccccattcaa
     1801 cagctggtga cggggcagtc tggcctcttt actcagtaca acatacagaa gaaagccatg
     1861 actgttcgag agttccgcaa gatagccaat agcgataagt actgtacccc acgctatagt
     1921 gagtttgaag agctcgagcg gaaatactgg aaaaatctta cattcaatcc tccaatctat
     1981 ggtgcagatg tgaatggtac cctctatgaa aagcatgttg atgagtggaa tattggccgg
     2041 ctgagaacaa tcctggactt ggtggaaaag gagagtggga tcaccattga gggtgtgaac
     2101 accccatacc tgtactttgg catgtggaag acatcctttg cttggcacac tgaagacatg
     2161 gacctctaca gcatcaacta cctgcacttt ggagaaccaa agtcctggta ctctgttcca
     2221 cctgagcatg gaaagcggtt ggaacgcctc gccaaaggct ttttcccagg aagtgctcaa
     2281 agctgtgagg catttctccg ccacaagatg accctgattt ccccgttaat gctgaagaaa
     2341 tatggaattc cctttgacaa ggtgactcaa gaggctggag agtttatgat cactttccct
     2401 tatggttacc atgccggctt taaccatggt tttaactgtg cggagtctac caattttgct
     2461 acccgtcggt ggattgagta cggcaagcaa gctgtgctgt gctcctgtag aaaggacatg
     2521 gtgaagatct ccatggatgt gtttgtgaga aagttccagc cagaaaggta caaactttgg
     2581 aaagctggga aggacaacac agttattgac catactctgc ccacgccaga agcagctgag
     2641 tttcttaagg agagtgaact gcctccaaga gctggcaacg aggaggagtg cccagaggag
     2701 gacatggaag gggtggagga tggagaggaa ggagacctga agacaagcct ggccaagcac
     2761 cgaataggga caaagaggca ccgagtttgt cttgaaatac cacaggaggt gagtcagagt
     2821 gagctcttcc ccaaggagga tctgagttct gagcagtatg agatgacgga gtgcccggca
     2881 gccctcgccc ctgtgaggcc cacccatagc tctgtgcggc aagttgagga tggtcttacc
     2941 ttcccagatt attctgactc cactgaagtc aaatttgaag agcttaaaaa tgtcaaacta
     3001 gaagaggagg atgaggagga agaacaagaa gcagctgcct tggatctttc tgtgaatcct
     3061 gcgtctgtag ggggacgcct tgtcttctca ggctccaaaa agaaatcatc ttctagcctg
     3121 ggctctggct cttcacggga ttctatctct tctgattcag aaactagtga gcctctctcc
     3181 tgccgagccc aagggcaaac gggagttctc actgtgcaca gttatgccaa aggggatggc
     3241 agggtcactg tgggagagcc atgcacgagg aagaaaggaa gcgccgctag aagtttcagt
     3301 gagcgggagc tggcagaggt tgcagatgaa tacatgtttt ccctagaaga gaataagaag
     3361 tccaagggac gccgtcagcc tttaagcaag ctcccccgcc atcacccact tgtgctgcag
     3421 gagtgtgtca gtgatgatga gacatctgaa cagctgaccc ctgaggaaga ggctgaggag
     3481 acagaggcct gggccaagcc tctgagccaa ctgtggcaga accgacctcc aaactttgag
     3541 gctgagaagg aattcaatga gaccatggcc caacaggccc ctcactgcgc tgtctgtatg
     3601 atcttccaga cttatcatca ggttgaattt ggaggcttta atcagaactg tggaaatgct
     3661 tcagatttag ccccccagaa gcagaggacc aagccattga ttccagaaat gtgcttcact
     3721 tcgactggct gcagcacgga catcaacctt tctactcctt atcttgagga ggatggcacc
     3781 agcatactcg tttcctgcaa gaagtgcagc gtccgggtcc atgccagttg ctatggggtc
     3841 ccccctgcaa aggcttctga agactggatg tgttctcggt gttcagccaa tgccctagag
     3901 gaggactgct gtttatgctc attacgagga ggggccctgc agagagcaaa tgatgacagg
     3961 tgggtccacg tttcatgtgc tgtggcaatt ctggaagcaa ggtttgtcaa cattgcagaa
     4021 agaagtccgg tggatgtgag caaaatcccc ctgccccgct tcaaactgaa atgtatcttc
     4081 tgtaagaagc ggaggaaaag aactgctggc tgctgtgtgc agtgttctca cggccgctgc
     4141 ccaactgcct tccatgtgag ctgcgcccag gctgccggtg tgatgatgca gcctgacgac
     4201 tggccttttg tggtcttcat tacctgcttt cggcacaaga ttcctaattt ggagcgtgcc
     4261 aagggggcct tgcaaagcat cactgcaggc cagaaagtca ttagcaagca taagaacggg
     4321 cgcttctacc agtgtgaagt ggtcaggctc accaccgaga ccttctatga agtcaacttt
     4381 gatgatggct ccttcagcga caatctttat cctgaggaca tagtgagcca ggactgtctc
     4441 cagtttggtc ctcctgctga aggggaagtg gtccaagtga gatggacaga cggccaagtc
     4501 tatggagcca agtttgtggc ctcccaccct atccaaatgt accaggtgga gtttgaggat
     4561 ggctcacaac ttgtggttaa gagagatgat gtatacacac tggatgaaga gcttcccaag
     4621 agagtcaaat ctagactgtc agtagcctca gacatgcgct tcaatgagat tttcacagag
     4681 aaagaggtta agcaagaaaa gaaacggcaa cgagttatca actcaagata ccgggaagat
     4741 tatattgagc ctgcactata ccgggccatc atggagtagg tgcttccagg gtccaaggga
     4801 ttctcagcca tccaggcaag agcactctgg gttccaaggg cgaattcgac ccagctttct
     4861 tgtacaaagt ggttgatgat ccgtcgagga attcactcct caggtgcagg ctgcctatca
     4921 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     4981 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     5041 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     5101 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     5161 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     5221 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     5281 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     5341 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     5401 tcttctctta tggagatccc tcgacggatc ggccgcaatt cgtaatcatg tcatagctgt
     5461 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5521 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5581 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5641 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5701 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5761 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5821 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5881 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5941 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6001 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6061 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6121 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6181 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6241 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6301 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6361 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6481 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6541 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6601 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6661 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6721 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6781 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6841 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6901 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6961 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7021 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7081 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7141 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7201 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7261 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7321 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7381 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7441 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7561 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7621 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     7681 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7741 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7801 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7861 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     7921 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     7981 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     8041 cacgacgttg taaaacgacg gccagtgcca agcttcacgc tgccgcaagc actcagggcg
     8101 caagggctgc taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc
     8161 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     8221 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     8281 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     8341 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatcctgctt
     8401 catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca
     8461 tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa
     8521 cacgcagatg cagtcggggc ggcgcggtcc caggtccact tcgcatatta aggtgacgcg
     8581 tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc
     8641 gcagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     8701 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     8761 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     8821 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     8881 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     8941 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     9001 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     9061 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     9121 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     9181 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     9241 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     9301 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     9361 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     9421 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     9481 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     9541 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9601 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccggga gatgggggag
     9661 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     9721 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     9781 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     9841 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagc
//
