LOCUS       sequence-189878-        7544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Hu H, Wang B, Borde M, Nardone J, Maika S, Allred L, Tucker PW, Rao 
            A
  TITLE     Foxp1 is an essential transcriptional regulator of B cell 
            development.
  JOURNAL   Nat Immunol. 2006 Aug . 7(8):819-26.
  PUBMED    16819554
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Immunol. 2006 Aug . 7(8):819-26."
COMMENT     Sequence Label: pcDNA3.1 Foxp1A
FEATURES             Location/Qualifiers
     source          1..7544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             924..950
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3099..3117)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3100..3117)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3137..3154)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3143..3367
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3413..3841
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3500..3519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3710..3731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3850..3870)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3855..4184
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4035..4170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4097..4116
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4251..5045
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4305..4324)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4915..4934
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5219..5340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5256..5275)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5310..5329
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5389..5405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5389..5405)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5402..5424)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5413..5429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5437..5443,5444..5461,5462..5467))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5482..5503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5620..5637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5791..6379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5871..5890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6550..7341,7342..7410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7173..7192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7411..7515)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta
      901 ccgagctcgg atccaccatg ggttatccat atgatgttcc agattatgct atgcaagaat
      961 ctgggtctga gacaaaaagt aacggatcgg ccatccagaa cgggtccagc ggtggcaacc
     1021 acttactaga gtgcggggca cttcgtgaca ctcggtccaa cggagaggca ccagcggtgg
     1081 acctgggggc agccgacctt gcccacgtcc agcagcagca acagcaggcc ctgcaggtgg
     1141 caaggcagct cctccttcag cagcagcaac agcagcagca gcaacagcag cagcagcagc
     1201 agcagcaaca gcagcaacag cagcagcaac aacagcagca gcagcaacag cagcagcagc
     1261 agcagcaagt tagtggatta aagtctccca agaggaatga caagcaacca gctcttcagg
     1321 ttcccgtgtc agtggctatg atgacacctc aggttatcac tcctcaacaa atgcagcaga
     1381 tcctccagca gcaggtgctg agcccccagc agctccaggt tctcctccag cagcagcagg
     1441 ccctcatgct tcaacagcag cagcttcaag aattttataa aaaacaacag gaacagttgc
     1501 agcttcaact tctccaacag caacatgctg gaaaacagcc gaaagagcag cagcaggtgg
     1561 ctacccagca gttggctttc cagcagcagc ttctccagat gcagcagctg cagcagcagc
     1621 acctcttgtc tctccagcgc caaggcctcc taacaattca gccggggcag cctgcccttc
     1681 ccctccaacc cctcgctcaa ggcatgattc caacggaact gcagcagctc tggaaagaag
     1741 tgacaagtgc ccacactgca gaggaaacca caagcagcaa ccacagcagc ctagacctga
     1801 ccagcacatg tgtctcgtcc tcggcacctt ccaagtcctc cctaatcatg aacccgcatg
     1861 cctctaccaa tggacagctc tcggtccaca ctcccaaaag ggaaagcttg tcccacgagg
     1921 agcaccccca cagccaccct ctctatggac atggcgtatg caagtggcca ggctgtgagg
     1981 cggtttgtga cgacttccca gcctttctaa aacatctcaa cagtgagcat gcgctggacg
     2041 atagaagcac agctcaatgt agagtacaaa tgcaggttgt acagcagtta gagctacagc
     2101 ttgcaaaaga caaagagcgc ctgcaagcca tgatgaccca cctgcatgtg aagtctacag
     2161 aacccaaagc tgcccctcag cccctgaatc tggtatcaag tgtcaccctc tccaagtctg
     2221 cctcagaggc ttctccacag agcttacctc atactccaac aacccccacc gcccccctga
     2281 ctcctgtcac ccaaggcccc tccgtcatca ccaccaccag catgcacacg gtgggaccta
     2341 tccgcaggcg gtactcagac aaatacaacg tgcccatttc ttcagcagat attgcgcaga
     2401 accaagaatt ttataagaac gcggaagtta gaccaccatt tacatatgca tctttaatca
     2461 ggcaggccat tctcgaatct ccagaaaagc agctaacact aaacgaaatc tataactggt
     2521 tcacacgaat gtttgcttac ttccgacgca atgcagccac gtggaagaat gcagtgcgtc
     2581 ataatcttag tctccacaag tgttttgtgc gagtagagaa cgttaaaggg gcagtatgga
     2641 cagtggatga agtagagttc caaaaacgga ggccacaaaa gatcagtggt aacccttccc
     2701 ttattaaaaa catgcagagc agccacgcct actgcacacc tctcaatgca gctttacagg
     2761 cttccatggc tgagaatagt atacctctgt acactaccgc ttccatggga aatcccactc
     2821 tgggcagcct ggccagtgcc atccgggagg agctgaacgg ggccatggag cacaccaaca
     2881 gcaacgagag tgacagcagt ccaggcagat cccctatgca agctgtgcac cccatacacg
     2941 tcaaagaaga acccctcgac cccgaggaag ctgaaggccc tctgtcctta gtgacaacag
     3001 ccaaccacag tccagatttt gaccatgaca gagattacga agacgaacca gtaaatgagg
     3061 acatggagtg actcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
     3121 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3181 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3241 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3301 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3361 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     3421 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3481 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3541 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3601 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3661 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3721 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3781 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3841 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3901 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     3961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4021 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4081 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4141 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4201 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4261 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4321 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4381 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4441 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4501 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4561 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4621 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4681 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4741 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     4801 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4861 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4921 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4981 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5041 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5101 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5161 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5221 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5281 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5341 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5401 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5461 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5521 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5581 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5641 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5701 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5761 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5821 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5881 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5941 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6001 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6061 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6361 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6421 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6481 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6541 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6601 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6661 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6721 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6781 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6841 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6901 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6961 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7021 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7081 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7141 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7201 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7261 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7321 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7381 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7441 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7501 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacg
//
