LOCUS       sequence-18992-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Brownawell AM, Macara IG
  TITLE     Exportin-5, a novel karyopherin, mediates nuclear export of 
            double-stranded RNA binding proteins.
  JOURNAL   J Cell Biol. 2002 Jan 7. 156(1):53-64.
  PUBMED    11777942
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Jan 7. 156(1):53-64."
COMMENT     Sequence Label: pQE60-Exp5
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(55..69,70..75,76..92,93..99)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa
       61 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
      121 caatttcaca cagaattcat taaagaggag aaattaacca tggcgatgga tcaagtaaac
      181 gcgctgtgcg agcagctggt gaaagcggtg acggtcatga tggaccccaa ctccacccag
      241 cgctaccggc tggaagccct caagttttgt gaggagttta aagaaaagtg tcctatctgt
      301 gtcccctgtg gcttgaggtt ggctgagaaa acacaagttg ccatcgtcag acattttggc
      361 cttcagatcc tggaacacgt tgtcaagttt cggtggaacg gcatgtctcg attggagaag
      421 gtgtatctga agaacagtgt catggagctg attgcaaatg gaacattgaa cattttggaa
      481 gaggagaacc atattaaaga tgctctgtct cgaattgtag tggaaatgat caagcgagag
      541 tggccacagc attggcctga catgctaata gaattggaca ctctttccaa acaaggggaa
      601 acacagacag aattggtgat gtttatcctt ttgcgactgg cagaggatgt agtgactttt
      661 cagacacttc cccctcaaag aagaagggac atccagcaaa cattaaccca gaacatggaa
      721 aggatcttca gttttctgct taacacactt caagaaaatg taaacaagta tcagcaagtg
      781 a
//
